Dr. James W. Tunnell Profile

Dr. James W. Tunnell

Professor at Univ of Texas at Austin

SPIE Involvement:

Conference Chair | Editor | Author | Student Chapter Advisor

Publications (55)

Proceedings Article | 13 March 2024 Presentation + Paper

Presentation + Paper

Hydrogel surface enhanced Raman spectroscopy sensor for monitoring prostate cancer

Jason King, Sanidhya Tripathi, Mengkun Chen, Mohadeseh Hashemi, M. Relvas, Marta Aranda-Palomer, Sergio Quintero, A. C. Oliveira, D. Costa, M. Correia, R. Sousa, Olga Martinho, Adriana Mendes, Fátima Baltazar, L. Dieguez, S. Abalde-Cela, James Tunnell

Proceedings Volume 12831, 128310F (2024) https://doi.org/10.1117/12.3002752

KEYWORDS: Hydrogels, Surface enhanced Raman spectroscopy, Prostate cancer, Plasma, Cancer, Sensors, Raman spectroscopy, Principal component analysis, Machine learning, Signal intensity

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Proceedings Article | 13 March 2024 Presentation + Paper

Presentation + Paper

Virtual H&E staining with single channel reflectance confocal microscopy images using pixel-to-pixel-based deep learning

Mengkun Chen, Matthew Fox, Jason Reichenberg, Fabiana C. P. Lopes, Katherine Sebastian, Mia Markey, James Tunnell

Proceedings Volume 12831, 1283105 (2024) https://doi.org/10.1117/12.3001974

KEYWORDS: Education and training, Adaptive optics, Reflectivity, Confocal microscopy, Skin, Aluminum, Tissues, Performance modeling, Image processing, Deep learning

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Proceedings Article | 13 March 2024 Presentation + Paper

Presentation + Paper

Evaluation of prostate cancer biosensor using Raman spectroscopy for remote monitoring applications

Sanidhya Tripathi, Jason King, Mengkun Chen, Mohadeseh Hashemi, Maria da Silva Relvas, Marta Aranda Palomer, Sergío Quintero-Moreno, Ana Oliveira, Diego Costa, Maria Clara Correia, Aureliana Sousa, Rui Sousa, Olga Martinho, Adriana Mendes, Fátima Baltazar, Lorena Díeguez Moure, Sara Abalde Cela, James Tunnell

Proceedings Volume 12831, 128310E (2024) https://doi.org/10.1117/12.3002021

KEYWORDS: Hydrogels, Cancer, Raman spectroscopy, Prostate cancer, Surface enhanced Raman spectroscopy, Biosensors, Sensors, Principal component analysis, Mouse models, Machine learning

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Proceedings Article | 12 March 2024 Presentation + Paper

Presentation + Paper

Multi-exposure speckle imaging through an optical fiber bundle

Logan Parker, Qingwei Fang, Andrew Dunn, James Tunnell

Proceedings Volume 12856, 1285604 (2024) https://doi.org/10.1117/12.3003394

KEYWORDS: Printed circuit board testing, Free space, Microfluidics, Laser speckle contrast imaging, Speckle, Beam path, Speckle pattern, Speckle imaging, Optical fibers, Cameras

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SPIE Journal Paper | 28 February 2023 Open Access

Open Access

Tissue harvest with a laser microbiopsy (Erratum)

Jason King, Nitesh Katta, Sapun Parekh, Thomas Milner, James Tunnell

JBO, Vol. 28, Issue 02, 029801, (February 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.2.029801

KEYWORDS: Tissues, Laser cutting, Plasma, Medical laser equipment, Laser ablation, Biomedical engineering

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SPIE Journal Paper | 14 December 2022 Open Access

Open Access

Tissue harvest with a laser microbiopsy

Jason B. King, Nitesh Katta, Sapun H. Parekh, Thomas E. Milner, James W. Tunnell

JBO, Vol. 27, Issue 12, 125001, (December 2022) https://doi.org/10.1117/12.10.1117/1.JBO.27.12.125001

KEYWORDS: Tissues, Biopsy, Pulsed laser operation, Skin, Laser ablation, Natural surfaces, Beam diameter, Biological research, Kidney, Cryogenics

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SPIE Journal Paper | 30 June 2022 Open Access

Open Access

Deep learning on reflectance confocal microscopy improves Raman spectral diagnosis of basal cell carcinoma

Mengkun Chen, Xu Feng, Matthew Fox, Jason Reichenberg, Fabiana C. P. Lopes, Katherine Sebastian, Mia Markey, James Tunnell

JBO, Vol. 27, Issue 06, 065004, (June 2022) https://doi.org/10.1117/12.10.1117/1.JBO.27.6.065004

KEYWORDS: Raman spectroscopy, Remote sensing, Tumor growth modeling, Confocal microscopy, Reflectivity, RGB color model, Tissues, Data modeling, Performance modeling, Tumors

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Proceedings Article | 7 March 2022 Presentation + Paper

Presentation + Paper

Laser microbiopsy with virtual H&E imaging for rapid minimally invasive diagnosis

Jason King, Nitesh Katta, Thomas Milner, James Tunnell

Proceedings Volume 11949, 1194905 (2022) https://doi.org/10.1117/12.2608931

KEYWORDS: Tissues, Laser tissue interaction, Adaptive optics, Reflectivity, Skin, Confocal microscopy, Natural surfaces, Biopsy, Glasses, Tissue optics

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Proceedings Article | 2 March 2022 Paper

Paper

Increased ablation efficiency in hard and soft tissues using an annular beam

Jason King, Nitesh Katta, Joel M. Teichman, James Tunnell, Thomas Milner

Proceedings Volume 11958, 119580E (2022) https://doi.org/10.1117/12.2608952

KEYWORDS: Tissues, Laser ablation, Beam shaping, Natural surfaces, Kidney, Laser therapeutics, Tissue optics, Soft tissue optics, Optical coherence tomography, Modulation

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SPIE Journal Paper | 23 September 2021 Open Access

Open Access

Imaging sub-diffuse optical properties of cancerous and normal skin tissue using machine learning-aided spatial frequency domain imaging

Andrew Stier, Will Goth, Aislinn Hurley, Treshayla Brown, Xu Feng, Yao Zhang, Fabiana C. P. Lopes, Katherine Sebastian, Pengyu Ren, Matthew Fox, Jason Reichenberg, Mia Markey, James Tunnell

JBO, Vol. 26, Issue 09, 096007, (September 2021) https://doi.org/10.1117/12.10.1117/1.JBO.26.9.096007

KEYWORDS: Optical properties, Tissue optics, Tissues, Skin, Data modeling, Spatial frequencies, Surgery, Monte Carlo methods, Optical imaging, Reflectivity

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SPIE Journal Paper | 17 March 2021 Open Access

Open Access

Machine learning to extract physiological parameters from multispectral diffuse reflectance spectroscopy

Mayna Nguyen, Yao Zhang, Frank Wang, Jose De La Garza Evia Linan, Mia Markey, James Tunnell

JBO, Vol. 26, Issue 05, 052912, (March 2021) https://doi.org/10.1117/12.10.1117/1.JBO.26.5.052912

KEYWORDS: Machine learning, Data modeling, Monte Carlo methods, Diffuse reflectance spectroscopy, MATLAB, Tissue optics, Reflectivity, Scattering, Optical properties, Tissues

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Proceedings Article | 5 March 2021 Presentation + Paper

Presentation + Paper

Characterization of laser microbiopsy for sub-microliter tissue sampling

Jason King, Nitesh Katta, James Tunnell, Thomas Milner

Proceedings Volume 11631, 1163111 (2021) https://doi.org/10.1117/12.2577258

KEYWORDS: Laser tissue interaction, Tissues, Tissue optics, Ray tracing, Diagnostics, Beam shaping, Aspheric lenses, Zemax, Thermal modeling

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Proceedings Article | 5 March 2021 Presentation + Paper

Presentation + Paper

Machine learning and the Gegenbauer Kernel improve mapping of sub-diffuse optical properties in the spatial frequency domain

Andrew Stier, Will Goth, Aislinn Hurley, Xu Feng, Yao Zhang, Fabiana C. P. S. Lopes, Katherine Sebastian, Matthew Fox, Jason Reichenberg, Mia Markey, James Tunnell

Proceedings Volume 11625, 1162509 (2021) https://doi.org/10.1117/12.2577324

KEYWORDS: Optical properties, Machine learning, Tissue optics, Data modeling, Computer simulations, Tissues, Statistical modeling, Skin, Image-guided intervention

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Proceedings Article | 5 March 2021 Presentation + Paper

Presentation + Paper

Optical monitoring the dynamics of plasmonic nanobubbles

Hieu T. Nguyen, Nitesh Katta, Thomas Milner, James Tunnell

Proceedings Volume 11661, 116610F (2021) https://doi.org/10.1117/12.2577896

KEYWORDS: Plasmonics, Optical coherence tomography, Interfaces, Laser therapeutics, Ultrafast measurement systems, Ultrafast imaging, Photography, Optical testing, Optical scanning systems, Nd:YAG lasers

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SPIE Journal Paper | 10 February 2021

Toward automated assessment of mole similarity on dermoscopic images

Yao Zhang, Kamil Ali, Jacob George, Jason Reichenberg, Matthew Fox, Adewole Adamson, James Tunnell, Mia Markey

JMI, Vol. 8, Issue 01, 014506, (February 2021) https://doi.org/10.1117/12.10.1117/1.JMI.8.1.014506

KEYWORDS: Image segmentation, Skin, RGB color model, Detection and tracking algorithms, Visualization, Skin cancer, Melanoma, Algorithm development, Evolutionary algorithms, Image processing algorithms and systems

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Purpose: Current skin cancer detection relies on dermatologists’ visual assessments of moles directly or dermoscopically. Our goal is to show that our similarity assessment algorithm on dermoscopic images can perform as well as a dermatologist’s assessment. Approach: Given one target mole and two other moles from the same patient, our model determines which mole is more similar to the target mole. Similarity was quantified as the Euclidean distance in a feature space designed to capture mole properties such as size, shape, and color. We tested our model on 18 patients, each of whom had at least five moles, and compared the model assessments of mole similarity with that of three dermatologists. Fleiss’ Kappa agreement coefficients and iteration tests were used to evaluate the agreement in similarity assessment among dermatologists and our model. Results: With the selected features of size, entropy (color variation), and cluster prominence (asymmetry), our algorithm’s similarity assessments agreed moderately with the similarity assessments of dermatologists. The mean Kappa of 1000 iteration tests was 0.49 (confidence interval ( CI ) = [ 0.23 , 0.74 ] ) when comparing three dermatologists and our model, which is comparable to the agreement in similarity assessment among the dermatologists themselves (the mean Kappa of 1000 iteration tests for three dermatologists was 0.48, CI = [ 0.19 , 0.77 ] .) By contrast, the mean Kappa was 0.22 (CI = [ − 0.00 , 0.43 ] ) when comparing the similarity assessments of three dermatologists and random guesses. Conclusions: Our study showed that our image feature-engineering-based algorithm can effectively assess the similarity of moles as dermatologists do. Such a similarity assessment could serve as the foundation for computer-assisted intra-patient evaluation of moles.

Proceedings Article | 19 February 2020 Presentation + Paper

Presentation + Paper

Rapid assessment of skin surgical margins using superpixel Raman spectroscopic imaging

Xu Feng, Matthew Fox, Jason Reichenberg, Fabiana C. P. Lopes, Katherine Sebastian, Andrew Dunn, Mia Markey, James Tunnell

Proceedings Volume 11222, 1122205 (2020) https://doi.org/10.1117/12.2546191

KEYWORDS: Raman spectroscopy, Confocal microscopy, Skin cancer, Optical spectroscopy imaging, Mohs surgery, Tissue optics

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SPIE Journal Paper | 11 May 2018 Open Access

Open Access

Raman biophysical markers in skin cancer diagnosis

Xu Feng, Austin Moy, Hieu T. Nguyen, Yao Zhang, Jason Zhang, Matthew Fox, Katherine Sebastian, Jason Reichenberg, Mia Markey, James Tunnell

JBO, Vol. 23, Issue 05, 057002, (May 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.5.057002

KEYWORDS: Raman spectroscopy, Collagen, Skin cancer, Tissues, Skin, Statistical modeling, Tumor growth modeling, Statistical analysis, Diagnostics, Remote sensing

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Proceedings Article | 13 February 2018 Paper

Paper

Raman spectroscopy reveals biophysical markers in skin cancer surgical margins

Xu Feng, Austin Moy, Hieu T. Nguyen, Yao Zhang, Matthew Fox, Katherine Sebastian, Jason Reichenberg, Mia Markey, James Tunnell

Proceedings Volume 10490, 104900B (2018) https://doi.org/10.1117/12.2288591

KEYWORDS: Raman spectroscopy, Tissues, Skin, Skin cancer, Mohs surgery, Tumors, Tissue optics, Confocal microscopy

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Proceedings Article | 16 February 2017 Presentation + Paper

Presentation + Paper

Interpreting fiber structure from polarization dependent optical anisotropy

Will Goth, Michael Sacks, James Tunnell

Proceedings Volume 10068, 100680P (2017) https://doi.org/10.1117/12.2252570

KEYWORDS: Anisotropy, Tissue optics, Structured optical fibers, Polarization, Light scattering, Birefringence, Collagen, Mie scattering, Refraction, Optical imaging, Spatial frequencies, Tissues, Data modeling, Scanning electron microscopy, Particles, Scattering

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Proceedings Article | 14 February 2017 Presentation + Paper

Presentation + Paper

Tumor margin assessment in Mohs surgery using reflectance, fluorescence and Raman spectroscopy

Hieu T. Nguyen, Austin Moy, Yao Zhang, Xu Feng, Jason Reichenberg, Matthew Fox, James Tunnell

Proceedings Volume 10054, 1005403 (2017) https://doi.org/10.1117/12.2253307

KEYWORDS: Fluorescence spectroscopy, Mohs surgery, Luminescence, Biopsy, Tissues, Raman spectroscopy, Diffuse reflectance spectroscopy, Skin cancer, Diagnostics, Pathology, Skin, Collagen, Tumors

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Proceedings Article | 9 February 2017 Presentation + Paper

Presentation + Paper

Spectral biopsy for skin cancer diagnosis: initial clinical results

Austin Moy, Xu Feng, Hieu Nguyen, Yao Zhang, Katherine Sebastian, Jason Reichenberg, James Tunnell

Proceedings Volume 10037, 1003704 (2017) https://doi.org/10.1117/12.2251293

KEYWORDS: Diffuse reflectance spectroscopy, Fluorescence spectroscopy, Raman spectroscopy, Clinical research, Biomedical engineering, Biopsy, Skin cancer, Spectroscopy, Data modeling, Fiber optics, Dermatology, Skin, Cancer, Luminescence, Melanoma, Data acquisition, Remote sensing

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Proceedings Article | 6 February 2017 Presentation + Paper

Presentation + Paper

Physiological basis for noninvasive skin cancer diagnosis using diffuse reflectance spectroscopy

Yao Zhang, Mia Markey, James Tunnell

Proceedings Volume 10037, 1003707 (2017) https://doi.org/10.1117/12.2253332

KEYWORDS: Skin cancer, Diffuse reflectance spectroscopy, Tumor growth modeling, Principal component analysis, Tissues, Monte Carlo methods, Data modeling, Scattering, Skin, Tissue optics, Cancer, Reflectivity

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SPIE Journal Paper | 20 May 2016 Open Access

Open Access

Color structured light imaging of skin

Bin Yang, John Lesicko, Austin Moy, Jason Reichenberg, Michael Sacks, James Tunnell

JBO, Vol. 21, Issue 05, 050503, (May 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.5.050503

KEYWORDS: Structured light, Stereolithography, Skin, Reflectivity, Scattering, Tissues, Color imaging, Imaging systems, Spatial frequencies, Pathology

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Proceedings Article | 27 April 2016 Presentation

Presentation

Clinical skin imaging using color spatial frequency domain imaging (Conference Presentation)

Bin Yang, John Lesicko, Austin Moy, Jason Reichenberg, James Tunnell

Proceedings Volume 9689, 96890B (2016) https://doi.org/10.1117/12.2211148

KEYWORDS: Skin, Absorption, Color imaging, Tissues, Scattering, Spatial frequencies, Imaging systems, Diagnostics, Optical properties, Dermatology

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Proceedings Article | 26 April 2016 Presentation

Presentation

Attenuation correction in molecular fluorescence imaging (Conference Presentation)

Bin Yang, James Tunnell

Proceedings Volume 9696, 969607 (2016) https://doi.org/10.1117/12.2211155

KEYWORDS: Signal attenuation, Luminescence, Absorption, Surgery, Tissues, In vitro testing, Tumors, Scattering, Optical calibration, Tissue optics

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Proceedings Article | 9 March 2016 Presentation + Paper

Presentation + Paper

Polarized spatial frequency domain imaging of heart valve fiber structure

Will Goth, Bin Yang, John Lesicko, Alicia Allen, Michael Sacks, James Tunnell

Proceedings Volume 9710, 971019 (2016) https://doi.org/10.1117/12.2212812

KEYWORDS: Tissues, Heart, Light scattering, Birefringence, Mueller matrices, Polarization, Polarization analysis, Anisotropy, Tissue optics, Imaging systems, Polarizers

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Proceedings Article | 7 March 2016 Presentation + Paper

Presentation + Paper

Biophysical basis for noninvasive skin cancer detection using Raman spectroscopy

Xu Feng, Austin Moy, Mia Markey, Matthew Fox, Jason Reichenberg, James Tunnell

Proceedings Volume 9704, 97040C (2016) https://doi.org/10.1117/12.2209421

KEYWORDS: Raman spectroscopy, Skin, Skin cancer, Tissues, Confocal microscopy, Optical fibers, Spectroscopy, Tissue optics, In situ metrology, Tumor growth modeling, Collagen

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Proceedings Article | 29 February 2016 Presentation + Paper

Presentation + Paper

Noninvasive skin cancer diagnosis using multimodal optical spectroscopy

Austin Moy, Xu Feng, Mia Markey, Jason Reichenberg, James Tunnell

Proceedings Volume 9689, 968905 (2016) https://doi.org/10.1117/12.2211172

KEYWORDS: Skin cancer, Biopsy, Skin, Tissue optics, Optical spectroscopy, Cancer, Spectroscopy, Optical design, Raman spectroscopy, Fluorescence spectroscopy, Spectrometers, Luminescence, Diffuse reflectance spectroscopy, Remote sensing, Light sources

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SPIE Journal Paper | 3 February 2015 Open Access

Open Access

Impact of one-layer assumption on diffuse reflectance spectroscopy of skin

Ricky Hennessy, Mia Markey, James Tunnell

JBO, Vol. 20, Issue 02, 027001, (February 2015) https://doi.org/10.1117/12.10.1117/1.JBO.20.2.027001

KEYWORDS: Skin, Diffuse reflectance spectroscopy, Absorption, Tissue optics, Scattering, Magnesium, Refractive index, Reflectivity, Packaging, Tissues

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SPIE Journal Paper | 6 November 2014 Open Access

Open Access

Clinical study of noninvasive in vivo melanoma and nonmelanoma skin cancers using multimodal spectral diagnosis

Liang Lim, Brandon Nichols, Michael Migden, Narasimhan Rajaram, Jason Reichenberg, Mia Markey, Merrick Ross, James Tunnell

JBO, Vol. 19, Issue 11, 117003, (November 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.11.117003

KEYWORDS: Skin, Melanoma, Laser induced fluorescence, Diagnostics, Raman spectroscopy, Remote sensing, Skin cancer, In vivo imaging, Tissues, Proteins

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SPIE Journal Paper | 27 October 2014 Open Access

Open Access

Effect of probe geometry and optical properties on the sampling depth for diffuse reflectance spectroscopy

Ricky Hennessy, Will Goth, Manu Sharma, Mia Markey, James Tunnell

JBO, Vol. 19, Issue 10, 107002, (October 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.10.107002

KEYWORDS: Optical properties, Scattering, Monte Carlo methods, Absorption, Anisotropy, Photons, Diffuse reflectance spectroscopy, Tissue optics, Reflectivity, Light scattering

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SPIE Journal Paper | 24 September 2014 Open Access

Open Access

Real-time absorption reduced surface fluorescence imaging

Bin Yang, James Tunnell

JBO, Vol. 19, Issue 09, 090505, (September 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.9.090505

KEYWORDS: Luminescence, Absorption, Spatial frequencies, Tissues, Image processing, Video, Tumors, Image acquisition, Visualization, Natural surfaces

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SPIE Journal Paper | 16 August 2013 Open Access

Open Access

Attenuation-corrected fluorescence extraction for image-guided surgery in spatial frequency domain

Bin Yang, Manu Sharma, James Tunnell

JBO, Vol. 18, Issue 08, 080503, (August 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.8.080503

KEYWORDS: Luminescence, Tissues, Absorption, Tumors, Image-guided intervention, Scattering, Optical properties, Signal attenuation, Reflectivity, In vitro testing

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Proceedings Article | 8 March 2013 Paper

Paper

Attenuation corrected fluorescence extraction using spatial frequency domain imaging system

Bin Yang, Manu Sharma, Youmin Wang, James Tunnell

Proceedings Volume 8618, 861809 (2013) https://doi.org/10.1117/12.2004784

KEYWORDS: Luminescence, Signal attenuation, Reflectivity, Absorption, Optical properties, Tumors, Image-guided intervention, Imaging systems, Tissues, Scattering

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SPIE Journal Paper | 1 March 2013 Open Access

Open Access

Monte Carlo lookup table-based inverse model for extracting optical properties from tissue-simulating phantoms using diffuse reflectance spectroscopy

Ricky Hennessy, Sam Lim, Mia Markey, James Tunnell

JBO, Vol. 18, Issue 03, 037003, (March 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.3.037003

KEYWORDS: Monte Carlo methods, Optical properties, Tissues, Diffuse reflectance spectroscopy, Tumor growth modeling, Photons, Absorption, Reflectivity, Data modeling, Scattering

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SPIE Journal Paper | 20 April 2012 Open Access

Open Access

Performance of a lookup table-based approach for measuring tissue optical properties with diffuse optical spectroscopy

Brandon Nichols, Narasimhan Rajaram, James Tunnell

JBO, Vol. 17, Issue 5, 057001, (April 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.5.057001

KEYWORDS: Scattering, Absorption, Reflectivity, Tissue optics, Optical properties, Tissues, Calibration, Oxygen, Mie scattering, Anisotropy

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SPIE Journal Paper | 1 December 2011 Open Access

Open Access

Development of a noncontact diffuse optical spectroscopy probe for measuring tissue optical properties

Sheldon Bish, Narasimhan Rajaram, Brandon Nichols, James Tunnell

JBO, Vol. 16, Issue 12, 120505, (December 2011) https://doi.org/10.1117/12.10.1117/1.3662459

KEYWORDS: Tissue optics, Scattering, Reflectivity, Optical properties, Polarization, Specular reflections, Tissues, Optical fibers, Absorption, Natural surfaces

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SPIE Journal Paper | 1 July 2011 Open Access

Open Access

Changes in morphology and optical properties of sclera and choroidal layers due to hyperosmotic agent

Raiyan Zaman, Brandon Nichols, Henry Rylander, Tianyi Wang, James Tunnell, Ashley Welch, Narasimhan Rajaram

JBO, Vol. 16, Issue 07, 077008, (July 2011) https://doi.org/10.1117/12.10.1117/1.3599985

KEYWORDS: Sclera, Blood vessels, Optical properties, Blood, Absorption, Scattering, Eye, Tissue optics, Tissues, Phase modulation

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SPIE Journal Paper | 1 January 2011 Open Access

Open Access

Probe pressure effects on human skin diffuse reflectance and fluorescence spectroscopy measurements

Liang Lim, Brandon Nichols, Narasimhan Rajaram, James Tunnell

JBO, Vol. 16, Issue 1, 011012, (January 2011) https://doi.org/10.1117/12.10.1117/1.3525288

KEYWORDS: Skin, Absorption, Neck, Tissues, Diffuse reflectance spectroscopy, Scattering, Blood, Tissue optics, Luminescence, Collagen

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SPIE Journal Paper | 1 May 2010 Open Access

Open Access

Lookup-table method for imaging optical properties with structured illumination beyond the diffusion theory regime

Tim Erickson, Amaan Mazhar, David Cuccia, Anthony Durkin, James Tunnell

JBO, Vol. 15, Issue 03, 036013, (May 2010) https://doi.org/10.1117/12.10.1117/1.3431728

KEYWORDS: Optical properties, Modulation, Reflectivity, Scattering, Absorption, Optical spheres, Photons, Diffusion, Tissue optics, Optical imaging

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SPIE Journal Paper | 1 March 2009 Open Access

Open Access

Near-infrared narrow-band imaging of gold/silica nanoshells in tumors

Priyaveena Puvanakrishnan, Jaesook Park, Parameshwaran Diagaradjane, Jon Schwartz, Christopher Coleman, Kelly Gill-Sharp, Kristina Sang, J. Donald Payne, Sunil Krishnan, James Tunnell

JBO, Vol. 14, Issue 02, 024044, (March 2009) https://doi.org/10.1117/12.10.1117/1.3120494

KEYWORDS: Tumors, Near infrared, Tissues, Composites, Absorption, Gold, Visualization, Tissue optics, Particles, Blood

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SPIE Journal Paper | 1 March 2009 Open Access

Open Access

Re-evaluation of model-based light-scattering spectroscopy for tissue spectroscopy

Condon Lau, Obrad Scepanovic, Jelena Mirkovic, Sasha McGee, Chung-Chieh Yu, Stephen Fulghum, Michael Wallace, James Tunnell, Kate Bechtel, Michael Feld

JBO, Vol. 14, Issue 02, 024031, (March 2009) https://doi.org/10.1117/12.10.1117/1.3116708

KEYWORDS: Light scattering, Reflectivity, Tissue optics, Scattering, Spectroscopy, Diffuse reflectance spectroscopy, Tissues, Packaging, Model-based design, Absorption

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Proceedings Article | 28 February 2009 Paper

Paper

In vivo determination of optical properties and fluorophore characteristics of non-melanoma skin cancer

Narasimhan Rajaram, Dianne Kovacic, Michael Migden, Jason Reichenberg, Tri Nguyen, James Tunnell

Proceedings Volume 7161, 716102 (2009) https://doi.org/10.1117/12.807776

KEYWORDS: Skin, Luminescence, Optical properties, Diffuse reflectance spectroscopy, Collagen, Scattering, Skin cancer, Laser induced fluorescence, Tissues, Blood

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Diffuse optical spectroscopy (DOS) and laser-induced fluorescence (LIF) techniques have widely been used as noninvasive tools for early cancer detection in several organs including the cervix, oral cavity and gastrointestinal tract. Using a combined DOS/LIF approach, one can simultaneously measure the morphology and biochemical composition of tissue and use these features to diagnose malignancy. We report for the first time to our knowledge both the optical properties and native fluorophore characteristics of non-melanoma skin cancer in the UV-visible range. We collected in vivo diffuse reflectance and intrinsic fluorescence measurements from 44 skin lesions on 37 patients. The skin sites were further categorized into three groups of non-melanoma skin cancer according to histopathology: 1) pre-cancerous actinic keratosis 2) malignant squamous cell carcinoma (SCC) and 3) basal cell carcinoma (BCC). We used a custom-built probe-based clinical system that collects both white light reflectance and laser-induced fluorescence in the wavelength range of 350-700 nm. We extracted the blood volume fraction, oxygen saturation, blood vessel size, tissue microarchitecture and melanin content from diffuse reflectance measurements. In addition, we determined the native fluorophore contributions of NADH, collagen and FAD from laser-induced fluorescence for all groups. The scattering from tissue decreased with progression from clinically normal to precancerous actinic keratosis to malignant SCC. A similar trend was observed for clinically normal skin and malignant BCC. Statistically significant differences were observed in the collagen contributions, which were lower in malignant SCC and BCC as compared to normal skin. Our data demonstrates that the mean optical properties and fluorophore contributions of normal, benign and malignant nonmelanoma cancers are significantly different from each other and can potentially be used as biomarkers for the early detection of skin cancer.

Proceedings Article | 24 February 2009 Paper

Paper

Narrow band imaging of tumors using gold nanoshells

Priyaveena Puvanakrishnan, Jaesook Park, Parameshwaran Diagaradjane, Jon Schwartz, Chris Coleman, Kelly Gill-Sharp, Kristina Sang, J. Donald Payne, Sunil Krishnan, James Tunnell

Proceedings Volume 7169, 716912 (2009) https://doi.org/10.1117/12.809613

KEYWORDS: Tumors, Near infrared, Tissues, Gold, Absorption, Composites, Visualization, Tissue optics, Blood vessels, Blood

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Proceedings Article | 18 February 2009 Paper

Paper

Two-photon-induced photoluminescence imaging of gold nanoshell's tumor biodistribution

Jaesook Park, Arnold Estrada, Jon Schwartz, Parmeswaran Diagaradjane, Sunil Krishnan, Chris Coleman, J. Donald Payne, Andrew Dunn, James Tunnell

Proceedings Volume 7192, 71920T (2009) https://doi.org/10.1117/12.809620

KEYWORDS: Tumors, Satellite navigation systems, Luminescence, Gold, Tissues, Tissue optics, Cancer, Multiplexing, Particles, Microscopes

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Proceedings Article | 12 February 2009 Paper

Paper

Changes in morphology and optical properties of sclera due to hyperosmotic agent

Raiyan Zaman, Henry Rylander, Narasimhan Rajaram, Tianyi Wang, Nitin Asokan, James Tunnell, Ashley Welch

Proceedings Volume 7175, 71750D (2009) https://doi.org/10.1117/12.809701

KEYWORDS: Sclera, Optical properties, Scattering, Absorption, Blood vessels, Eye, Oxygen, Blood, Light scattering, Eye models

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The primary and the secondary goals of this study were to investigate the change in morphology and optical properties of sclera due to a hyperosmotic agent i.e. 100% anhydrous glycerol. We performed our experiments in vivo on the sclera of 8 rabbits and 3 miniature pigs. All the animals were under anesthetic for the entire experiment according to an approved protocol. The position of the eye was stabilized with a suture placed in the limbus. Glycerol was delivered to sclera in 2 methods (i) injection (using a hypodermic needle 27G ½), (ii) direct application after 0.3 cm incision at conjunctiva. A camera attached to a slit lamp was used to capture the morphological changes of the sclera. For the secondary goal we used a diffuse optical spectroscopy (DOS) system with a linear fiber arrangement to measure reflectance from the sclera before and after application of glycerol. The probe source-detector separation was set to 370 μm for optimal penetration depth. We fit the measured diffuse reflectance to a Lookup Table (LUT)-based inverse model specific to our probe geometry to determine the scattering and absorption properties of the sclera. This method estimated the size and density of scatterers, absorbers-blood volume fraction, melanin concentration, oxygen saturation, and blood vessel size. The results illustrated that the initial clearing of sclera started 3 minutes after injecting glycerol to sclera. The sclera became completely transparent at 8 minutes and stayed clear for 10-15 minutes. During this time the choroid layer was visible through sclera. The clear sclera became less transparent over next 11 minutes and became completely opaque once we applied 0.9% saline to hydrate the sclera. These dehydration and hydration cycles were repeated 4 times for each eye and the results were consistent for all animal models. When glycerol was applied directly to sclera after the incision at the conjunctiva, the sclera became transparent instantaneously. For the secondary goal, the changes in optical properties of sclera were monitored during the dehydration and hydration cycles. The reduced scattering coefficient decreased when glycerol was injected and it further reduced with direct application. The scattering increased after re-hydration. We also measured the blood volume fraction, melanin concentration, oxygen saturation, and blood vessels diameter to calculate absorption coefficient with the DOS system. This study provided a novel way to identify morphological changes of sclera in addition to measuring changes in optical properties due to hyper osmotic agent. The changes in optical properties were consistent with the morphological changes in sclera during the dehydration and hydration cycles.

SPIE Journal Paper | 1 September 2008 Open Access

Open Access

Lookup table–based inverse model for determining optical properties of turbid media

Narasimhan Rajaram, Tri Nguyen, James Tunnell

JBO, Vol. 13, Issue 05, 050501, (September 2008) https://doi.org/10.1117/12.10.1117/1.2981797

KEYWORDS: Tissue optics, Tissues, Tumor growth modeling, Optical properties, Diffuse reflectance spectroscopy, Absorption, Data modeling, Scattering, Reflectivity, Calibration

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Proceedings Article | 25 February 2008 Paper

Paper

Clinical instrument for spectral diagnosis of cutaneous malignancy

Narasimhan Rajaram, Timothy Aramil, Kelvin Lee, James Tunnell

Proceedings Volume 6848, 68480R (2008) https://doi.org/10.1117/12.759709

KEYWORDS: Luminescence, Tissues, Tissue optics, Absorption, Diffuse reflectance spectroscopy, Scattering, Reflectivity, Melanoma, Skin, Light scattering

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Proceedings Article | 22 February 2008 Paper

Paper

Gold nanoshell mediated hyperthermia enhances the efficacy of radiation therapy

Parmeswaran Diagaradjane, Anil Shetty, James Wang, Andrew Elliot, Jon Schwartz, Shujun Shentu, Chul Park, Amit Deorukhkar, Jason Stafford, Sang Cho, James Tunnell, John Hazle, Sunil Krishnan

Proceedings Volume 6865, 68650N (2008) https://doi.org/10.1117/12.764021

KEYWORDS: Tumors, Gold, Tissues, Radiotherapy, Magnetic resonance imaging, Temperature metrology, Magnetism, Thermography, Laser irradiation, Thermometry

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Proceedings Article | 21 February 2008 Paper

Paper

3D microscopy of gold nanoshells in tumors using two-photon-induced photoluminescence

Jaesook Park, Arnold Estrada, Jon Schwartz, J. Donald Payne, Andrew Dunn, James Tunnell

Proceedings Volume 6869, 68690L (2008) https://doi.org/10.1117/12.764123

KEYWORDS: Luminescence, Tumors, Gold, 3D image processing, Particles, 3D acquisition, Near infrared, Microscopy, Cancer, Silica

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Proceedings Article | 12 September 2003 Paper

Paper

Thermal response of human skin epidermis in different skin types to 595-nm laser irradiation and cryogen spray cooling: an ex-vivo study

Tianhong Dai, Brian Pikkula, James Tunnell, David Chang, Bahman Anvari

Proceedings Volume 4949, (2003) https://doi.org/10.1117/12.476134

KEYWORDS: Skin, Injuries, Blood vessels, Cryogenics, Laser irradiation, Pulsed laser operation, Laser therapeutics, In vivo imaging, Laser coagulation, Dye lasers

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Proceedings Article | 17 June 2002 Paper

Paper

In-vivo study of epidermal protection by cryogen spray cooling during pulsed-laser irradiation at high radiant exposures

James Tunnell, David Chang, Carol Johnston, Jorge Torres, Charles Patrick, Michael Miller, Sharon Thomsen, Bahman Anvari

Proceedings Volume 4609, (2002) https://doi.org/10.1117/12.431782

KEYWORDS: Skin, Cryogenics, Tissues, Laser therapeutics, Injuries, In vivo imaging, Pulsed laser operation, Laser damage threshold, Blood vessels, Blood

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Proceedings Article | 17 June 2002 Paper

Paper

RF nonablative cutaneous thermal therapy

James Tunnell, Roger Stern, Karl Pope

Proceedings Volume 4609, (2002) https://doi.org/10.1117/12.432073

KEYWORDS: Tissues, Skin, Cryogenics, Electrodes, 3D modeling, Absorption, Amplifiers, Copper, Natural surfaces, Mathematical modeling

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Proceedings Article | 17 June 2002 Paper

Paper

Cryogen spray cooling: effects of cryogen film on heat removal and light transmission

Brian Pikkula, Yacov Domankevitz, James Tunnell, Bahman Anvari

Proceedings Volume 4609, (2002) https://doi.org/10.1117/12.432078

KEYWORDS: Cryogenics, Skin, Pulsed laser operation, Laser therapeutics, Glasses, Temperature metrology, Laser systems engineering, Signal attenuation, Laser vision correction, In vitro testing

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Proceedings Article | 21 May 2001 Paper

Paper

Thermal and fluid characteristics during cryogen spray cooling

Bahman Anvari, Brian Pikkula, James Tunnell, Jorge Torres

Proceedings Volume 4244, (2001) https://doi.org/10.1117/12.427766

KEYWORDS: Cryogenics, Skin, Epoxies, Laser therapeutics, Temperature metrology, Injuries, Aluminum, Thermal effects, Chromophores, Heat flux

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Showing 5 of 55 publications

Proceedings Volume Editor (4)

SPIE Conference Volume | 4 April 2024

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXII

SPIE Conference Volume | 24 March 2023

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI

SPIE Conference Volume | 24 March 2022

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XX

SPIE Conference Volume | 16 April 2021

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIX

Conference Committee Involvement (9)

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXIII

25 January 2025 | San Francisco, California, United States

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXII

27 January 2024 | San Francisco, California, United States

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI

28 January 2023 | San Francisco, California, United States

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XX

23 January 2022 | San Francisco, California, United States

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIX

6 March 2021 | Online Only, California, United States

Showing 5 of 9 Conference Committees

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