Prof. Irina Vasilenko Profile

Prof. Irina Vasilenko

at A.N. Kosygin Russian State Univ

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Author

Publications (13)

Proceedings Article | 5 March 2021 Poster + Presentation + Paper

QPI and atomic force microscopy in a comparative evaluation of biopolymer composites for a biomimetic matrix

Irina Vasilenko, Vladislav Metelin, Nataliya Kil’deeva, Vasilina Zakharova, Nina Shikhina

Proceedings Volume 11653, 116531U (2021) https://doi.org/10.1117/12.2578741

KEYWORDS: Biopolymers, Composites, Atomic force microscopy, Biomimetics, Matrices, Biological research, Visualization, Tissues, Tissue engineering, Stem cells

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Biopolymers are actively used in tissue engineering for the production of biomedical products with desired and controlled properties. Particular attention is paid to the creation of an artificial biomimetic matrix - a framework for targeted regeneration, effective cell growth and their integration with the surrounding tissue. The purpose of the work is to identify the possibilities of interference microscopy for studying the structural, functional and biological properties of biopolymer matrices based on fibroin and chitosan. The analysis of the thickness, relief features, and structure of biopolymer matrices was carried out using methods of superresolving interference and atomic force microscopy. We used a MIM-340 laser modulation interference microscope (Shvabe, Russia) with following technical characteristics: resolution in the lateral plane - 13 nm; vertical resolution - 0.1 nm; the range of measurement of linear vertical dimensions - 0.01–0.10 μm, in the lateral plane (for a 20x lens) - 0.1–8.0 μm; field of view of the measuring channel in the lateral plane of not less than 20 microns. Atomic force microscopy was performed on the basis of the NtegraPrima micro-cantilever system (NT-MDT, Russia) in semi-contact mode. We visualized and quantified the relief and heterogeneity of the internal structure of biopolymer matrices in the obtained interferograms. The effect of the addition of fibroin and jenipine on the thickness, roughness, and structural uniformity of the composite matrix coating has been studied. To assess the biocompatibility, adhesive and immunogenic properties of chitosan and fibroin-containing polymers, populations of living cells (lymphocytes, neutrophils and mesenchymal stem cells) were used. High informativeness of the QPI in the study of the morpho-functional properties of films and nanofiber materials was established. Based on the results of a comparative analysis of new biopolymer composites, the most promising samples for creating medical devices with characteristics as close to physiological as possible are determined.

Proceedings Article | 1 April 2020 Paper

New approach to the study of cell cytotoxicity using high-resolution coherence phase-interference microscopy

Irina Vasilenko, Vladislav Metelin, Nataliya Kil’deeva, Andrey Temnov, Roman Lifenko, Nina Shikhina

Proceedings Volume 11359, 113591R (2020) https://doi.org/10.1117/12.2555958

KEYWORDS: Blood, Microscopy, Diagnostics, Microscopes, Control systems, Phase imaging, Holography, Mirrors, In vitro testing, Magnetism

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Proceedings Article | 22 July 2019 Paper

The potential of laser interferometry for a non-invasive assessment of biopolymer film structure and biological properties

Irina Vasilenko, Nataliya Kil'deeva, Vladislav Metelin, Nikita Sazhnev, Vasilina Zakharova, Nina Shikhina

Proceedings Volume 11076, 110761R (2019) https://doi.org/10.1117/12.2527207

KEYWORDS: Biopolymers, Polymers, Laser interferometry, Composites, Mirrors, Interferometry, Microscopy, Microscopes, Blood, Adhesives

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Proceedings Article | 19 July 2019 Paper

Modulation interference microscopy as a promising method for assessing the risk of metastasis in patients with breast cancer

Irina Vasilenko, Vladislav Metelin, Andrey Balkanov, Pavel Ignatiev

Proceedings Volume 11073, 1107322 (2019) https://doi.org/10.1117/12.2527200

KEYWORDS: Tumors, Modulation, Microscopy, Breast cancer, Blood, Cancer, Statistical analysis, Lymphatic system, Cell death, Diagnostics

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Proceedings Article | 4 March 2019 Paper

Evaluation of metastatic potential of circulating tumor cells using quantitative phase imaging (QPI)

Irina Vasilenko, Vladislav Metelin, Ziver Kardasheva, Andrey Balkanov, Roman Lifenko

Proceedings Volume 10887, 1088724 (2019) https://doi.org/10.1117/12.2510383

KEYWORDS: Tumors, Blood, Cancer, Phase imaging, Oncology, Cell death, Microscopes, Visualization, Molecules, Biological research

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Proceedings Article | 23 February 2018 Paper

Quantitative phase imaging of platelets in patients with chronic renal failure treated with hemodialysis

Irina Vasilenko, Elizaveta Vlasova, Vladislav Metelin, Ziver Kardasheva

Proceedings Volume 10503, 105031N (2018) https://doi.org/10.1117/12.2291416

KEYWORDS: Blood, Phase imaging, Kidney, Refractive index, Failure analysis, Microscopes, Statistical analysis, Collagen, Clinical research, Medicine

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Proceedings Article | 21 February 2017 Presentation + Paper

Opportunities of QPI in the epigenetic diagnostics and assessment of therapeutic efficacy

Irina Vasilenko, Vladislav Metelin, Aleksandr Kuznetsov, Vladimir Belyakov, Tatiana Yakushina

Proceedings Volume 10074, 1007417 (2017) https://doi.org/10.1117/12.2253033

KEYWORDS: Biosensors, Phase imaging, Proteins, Organisms, Sensors, Biological research, Medical research

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Proceedings Article | 16 February 2017 Paper

Quantitative phase imaging of platelet: assessment of cell morphology and function

Irina Vasilenko, Elizaveta Vlasova, Vladislav Metelin, B. Agadzhanjan, R. Lyfenko

Proceedings Volume 10068, 100681X (2017) https://doi.org/10.1117/12.2253160

KEYWORDS: Cancer, Phase imaging, Microscopy, Oncology, Image analysis, Tumors, Blood, Refractive index, Statistical analysis

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Proceedings Article | 11 March 2015 Paper

Quantitative phase imaging of cellular and subcellular structures for non-invasive screening diagnostics of socially significant diseases

Irina Vasilenko, Vladislav Metelin, Marat Nasyrov, Vladimir Belyakov, Alexander Kuznetsov, Evgeniy Sukhenko

Proceedings Volume 9336, 93362K (2015) https://doi.org/10.1117/12.2078661

KEYWORDS: Fluctuations and noise, Phase imaging, Diagnostics, Microscopy, Blood, Proteins, Real time imaging, Phase interferometry, Medical research, Interferometry

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Proceedings Article | 12 February 1999 Paper

Real-time monitoring of the functional status of platelets treated by Infukoll using a computer-aided laser phase microscope

Irina Vasilenko, Svetlana Babakova, Elena Kiseleva, Adyan Dyugeev, Alexander Konradov, Vladimir Shabalin

Proceedings Volume 3567, (1999) https://doi.org/10.1117/12.339179

KEYWORDS: Statistical analysis, Image processing, Microscopes, Blood, Visualization, Image analysis, 3D image processing, Image compression, Visual optics, Image visualization

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Combined analysis of optic-geometrical characteristics makes it possible to comprehensively evaluate the morphological and functional state of the cytological object, which can not be done during visual observation. The technique is discussed for real-time monitoring of the functional status of platelets using computer-aided phase microscope (CPM) 'Cytoscan'. High accuracy and sensitivity of CPM with respect to determination of local temporal phase make it possible to register the dynamic processes in the voluntarily chosen points and sections of micro-objects, to obtain the Fourier's spectra and other characteristics suitable for statistical analysis. Human platelets were prepared from venous blood of healthy donors and pregnant women by standard methods, suspended in culture medium 199 and treated by different doses of 6% Infukoll HES. Nonfixating and nonstaining cells were studied with CPM: height accuracy 0.5 nm, magnification 1000, acquisition time 4 - 30 s. In our experiments we used time resolution about 0.03 s and 30 x lens with numerical aperture 0.65. During investigations of temporal processes a certain section was chosen in the topogram of cell image and local values for the phase of scattered wave in each of the points of the chosen cell's profile were measured. On the basis of the results of automated phase image analysis of optic-geometrical characteristics of living cells, the new quantitative express-method for evaluating of the functional status of human platelets was developed and tested. The structural changes of cells were visualized in alteration of 3-D images, phase profiles, in the decrease of mean cell phase diameters, heights, volumes, in disturbance of histograms of phase heights distribution by cell image points. New data on the behavior of platelets treated by Infukoll in vitro and in vivo were obtained. Analysis of intracellular dynamics was allowed to characterize the cell's regions of maximal activity, but the intensity of processes taking place in these regions in accordance with frequencies as well. The difference between the calculated values of frequencies (from 0.1 to 17 H) is associated with the influence of Infukoll on the metabolism and functional activity of living platelets.

Proceedings Article | 15 January 1996 Paper

Influence of He-Ne laser blood irradiation on morphofunctional state of monocytes in asthmatic patients

N. Paleev, O. Slinchenko, V. Ilchenko, Irina Vasilenko, Alexander Konradov, Vladimir Tychinsky

Proceedings Volume 2630, (1996) https://doi.org/10.1117/12.230038

KEYWORDS: Blood, Chemiluminescence, Helium neon lasers, Microscopy, Microscopes, Oxygen, Osmium, Laser therapeutics, Medical research, Clinical research

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Proceedings Article | 15 January 1996 Paper

Real-time monitoring of the cell physiological and pathological processes using computer-aided phase microscope

Irina Vasilenko, Vladimir Shabalin, Vladimir Tychinsky, Georgy Kufal, A. Konradov, O. Slinchenko

Proceedings Volume 2628, (1996) https://doi.org/10.1117/12.230006

KEYWORDS: Pathology, Microscopes, Geometrical optics, Image processing, Blood, Super resolution, Quantitative analysis, Electronics, Medicine, Biology

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Proceedings Article | 1 February 1995 Paper

Real-time monitoring of immunology reactions using computer-aided phase microscope

Vladimir Tychinsky, Georgy Kufal, Alex Odintsov, Irina Vasilenko, Svetlana Babakova, Vladimir Shabalin

Proceedings Volume 2329, (1995) https://doi.org/10.1117/12.200898

KEYWORDS: Microscopes, Image processing, Blood, Super resolution, In vitro testing, Electronics, Mirrors, Geometrical optics, Mode conditioning cables, Gaussian beams

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Showing 5 of 13 publications

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