We present a novel optical microscope technique to detect the chromatin organization and DNA damage foci in the unlabeled live cell nuclei. The dynamic scattering signal of chromatin is recorded by a highly sensitive interference microscopy, contrast-enhanced coherent brightfield microscopy (COBRI), at a high speed. By analyzing the temporal fluctuations of the scattering signal, the density and the compaction level of chromatin are accurately estimated with the sub-micrometer spatial resolutions. This imaging strategy is referred to as “DYNAMICS imaging”. Using DYNAMICS imaging, we monitor the dynamic chromatin remodeling initiated by DNA damage.
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