Label-free visualization of chromatin remodeling in live cell nuclei by analyzing the dynamic interference scattering signal (Conference Presentation)

Yi-Teng Hsiao; Fasih Bintang Ilhami; I-Hsin Liao; Chia-Lung Hsieh

doi:10.1117/12.2646966

15 March 2023 Label-free visualization of chromatin remodeling in live cell nuclei by analyzing the dynamic interference scattering signal (Conference Presentation)

Yi-Teng Hsiao, Fasih Bintang Ilhami, I-Hsin Liao, Chia-Lung Hsieh

Author Affiliations +

Proceedings Volume PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; PC123850L (2023) https://doi.org/10.1117/12.2646966
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We present a novel optical microscope technique to detect the chromatin organization and DNA damage foci in the unlabeled live cell nuclei. The dynamic scattering signal of chromatin is recorded by a highly sensitive interference microscopy, contrast-enhanced coherent brightfield microscopy (COBRI), at a high speed. By analyzing the temporal fluctuations of the scattering signal, the density and the compaction level of chromatin are accurately estimated with the sub-micrometer spatial resolutions. This imaging strategy is referred to as “DYNAMICS imaging”. Using DYNAMICS imaging, we monitor the dynamic chromatin remodeling initiated by DNA damage.

Conference Presentation

Citation Download Citation

Yi-Teng Hsiao, Fasih Bintang Ilhami, I-Hsin Liao, and Chia-Lung Hsieh "Label-free visualization of chromatin remodeling in live cell nuclei by analyzing the dynamic interference scattering signal (Conference Presentation)", Proc. SPIE PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, PC123850L (15 March 2023); https://doi.org/10.1117/12.2646966

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