This paper introduces an optimized and affordable optofluidic add-on device for continuous and rapid light sheet imaging of C. elegans. Platform utilizes an optofluidic chip with integrated PDMS microlens to generate a light-sheet and rapidly images worms flowing in a microchannel via a regular microscope. Validation results on control and 6-OHDA-exposed worms demonstrates the ability of platform in qualitative and quantitative imaging of neuronal degenerations at different ages. The developed affordable platform enables a conventional fluorescent microscope to offer high-content and high-throughput light-sheet images of C. elegans populations which is invaluable to aging, neurodegeneration, and drug discovery studies.
This work reports on the development of a low-cost and compact optofluidic add-on device for converting a conventional wide-field microscope to a light sheet fluorescence microscope for immobilization-free imaging of the C. elegans model organism. The developed Polydimethylsiloxane (PDMS)-based optofluidic device consists of an integrated PDMS cylindrical lens for light-sheet generation and a microfluidics channel for flow-based translation of samples through the light sheet. Validation experiments on several strains of C. elegans demonstrate the ability of the device in volumetric imaging of the fluorescence expressions of entire worms in a few seconds, at the single-neuron resolution, and with high contrast.
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