Sandwich-based LFA device was successfully demonstrated to detect salivary lipopolysaccharides of P. gingivalis from human saliva. Although saliva is an attractive biofluid due to non-invasive sampling and excellent availability, isolating targeted biomarkers for analysis in saliva is challenging because of interferences from various biomolecules in saliva, especially by -amylase. Combined pre-treatment using potato starch and syringe filtration has been developed to reduce the interference from -amylase. Pretreated saliva presented a comparable LOD ~46 ng/mL with excellent selectivity versus other LPS and proteins. Future directions include not only the development of aptamer-based LFA but also adapting the Surface Enhanced Raman Scattering (SERS) technology for sensing target analytes in LFA in conjunction with novel silver-coated Au nanostar particles. Our prior research using aptamer in LFA and preliminary results of SERS detection in LFA will be presented to support our future directions.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.