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7 June 2024 Advanced point-of-care LFA devices to detect the salivary LPS of P. gingivalis bacteria for oral health
Daewoo Han, Lyndsay Kissall, Der Vang, Pietro Strobbia, Sancai Xie, Andrew J. Steckl
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Proceedings Volume PC13059, Smart Biomedical and Physiological Sensor Technology XXI; PC1305906 (2024) https://doi.org/10.1117/12.3012674
Event: SPIE Defense + Commercial Sensing, 2024, National Harbor, Maryland, United States
Abstract
Sandwich-based LFA device was successfully demonstrated to detect salivary lipopolysaccharides of P. gingivalis from human saliva. Although saliva is an attractive biofluid due to non-invasive sampling and excellent availability, isolating targeted biomarkers for analysis in saliva is challenging because of interferences from various biomolecules in saliva, especially by -amylase. Combined pre-treatment using potato starch and syringe filtration has been developed to reduce the interference from -amylase. Pretreated saliva presented a comparable LOD ~46 ng/mL with excellent selectivity versus other LPS and proteins. Future directions include not only the development of aptamer-based LFA but also adapting the Surface Enhanced Raman Scattering (SERS) technology for sensing target analytes in LFA in conjunction with novel silver-coated Au nanostar particles. Our prior research using aptamer in LFA and preliminary results of SERS detection in LFA will be presented to support our future directions.
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Daewoo Han, Lyndsay Kissall, Der Vang, Pietro Strobbia, Sancai Xie, and Andrew J. Steckl "Advanced point-of-care LFA devices to detect the salivary LPS of P. gingivalis bacteria for oral health", Proc. SPIE PC13059, Smart Biomedical and Physiological Sensor Technology XXI, PC1305906 (7 June 2024); https://doi.org/10.1117/12.3012674
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KEYWORDS
Point-of-care devices
Bacteria
Cardiovascular disorders
Neurological disorders
Interfaces
Nervous system
Particles
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