The difficulties identifying the underlying mechanisms in biomineralization are compounded by the lack of imaging techniques which simultaneously provide the desired chemical and elemental sensitivity to both identify sparse nanoscale organics and inorganic crystal structure across a large field of view. Here, we combine nanoscale secondary ion mass spectroscopy (nano-SIMS) and infrared scattering-scanning near-field optical microscopy (IR s-SNOM) to gain simultaneous elemental and chemical nano-images across 10’s of micrometer scales. We map the loss of order within the biomineralized material to gain insight in its formation processes whereby amorphous phases assemble into well-ordered macrostructures.
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