KEYWORDS: In vivo imaging, Super resolution, Nanoimaging, Image resolution, Microspheres, Evanescence, Spatial frequencies, Multimode fibers, Modulation, Imaging systems
Existing super-resolution imaging technology relies on rigid and bulky systems, which limits its application in narrow space. Endoscopy based on multimode fibers (MMF) has emerged as a significant solution for in vivo imaging. There is a high requirement to observe samples inside the lumen of the body simultaneously break through the diffraction limit. We propose the frequency-shift mechanism for universal super-resolution imaging with light-field encoded modulation. In this paper, we demonstrate our method for a single MMF in vivo imaging at subcellular resolution through light-field encoded, which can also ensure stable imaging under various operating conditions.
The main challenge in multimode fiber imaging is modal scrambling caused by environmental fluctuation. How to get high contrast and high stable imaging is the main question. In this presentation, we propose some methods to increase the contrast-to-noise ratio and stability of multimode fiber imaging. Wavelength modulation is introduced to suppress the background. Exhaustive bending effect was used to improve the imaging stability. Wavelength modulation is introduced to enhance the CNR four fold in a 200 μm field-of-view imaging. We show a near diffraction limited focusing capability at imaging depths of up to 150 µm with near constant lateral resolutions of 2.1 µm. The imaging of small fluorescent beads embedded in a 3D matrix was demonstrated.
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