A label-Free DNA Hybridization biosensor is proposed and demonstrated through a microfiber Bragg grating with self-assembly technique. By recording the wavelength separation between the two resonant peaks of a single mFBG, temperature-compensated biosensing measurement has been achieved to detect the concentrations of 1 μM the target ssDNA with high specificity.
A taper interferometer embedded in fiber Bragg grating (FBG) is proposed and experimentally demonstrated for labelfree detection of breast cancer biomarker (HER2). The tapered fiber-optic interferometer sensor is extremely sensitive to ambient refractive index (RI) and the resonant wavelength of FBG is essentially insensitive to the RI variation. The FBG can be applied as a temperature thermometer, which monitoring the undesired drifts due to its independent response to the temperature. The label-free bio-recognition scheme is achieved by the covalent immobilization method for conjugation with HER2 antibody to achieve target biomarker specific detection. The proposed sensor presents a low limit-of-detection (LOD) of 5 ng/mL, providing a platform for the application in early diagnosis on the breast cancer.
Microfiber Bragg gratings (mFBGs) can be used as cost-effective and relatively simple-to-implement biosensors for monitoring DNA interactions in situ. The sensors are functionalized by a monolayer of poly-L-lysine (PLL) with the specific molecular recognition probe DNA sequences to bind with high specificity to a given target. By recording the wavelength seperation between the two resonant peaks of a single mFBG, the mFBG biosensor is capable of detecting the presence of specific target DNA in situ.
We demonstrate an acid-based sensor from the biofuncationalized microfiber Bragg grating. By electrostatic selfassembly layer-by-layer technique, the film consisting of sodium alginate which has hygroscopic response to the potential of hydrogen is coated on the fiber surface. Consequently, the refractive index variation of the sensing film caused by water absorption can be measured by mFBG’s higher order mode peak which can be translated into pH value information. The sensitivity of the sensor is received as high as 265pm/pH.
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