Dr. Yingxiao Wang Profile

Dr. Yingxiao Wang

at Univ of Illinois

SPIE Involvement:

Author

Publications (3)

Proceedings Article | 14 March 2012 Paper

Molecular signaling in live cells studied by FRET

Shu Chien, Yingxiao Wang

Proceedings Volume 8329, 832902 (2012) https://doi.org/10.1117/12.919126

KEYWORDS: Fluorescence resonance energy transfer, Biosensors, Visualization, Plasma, Proteins, Cancer, Signaling molecules, Receptors, Live cell imaging, In vitro testing

Read Abstract +

Genetically encoded biosensors based on fluorescence resonance energy transfer (FRET) enables visualization of signaling events in live cells with high spatiotemporal resolution. We have used FRET to assess temporal and spatial characteristics for signaling molecules, including tyrosine kinases Src and FAK, small GTPase Rac, calcium, and a membrane-bound matrix metalloproteinase MT1-MMP. Activations of Src and Rac by platelet derived growth factor (PDGF) led to distinct subcellular patterns during cell migration on micropatterned surface, and these two enzymes interact with each other to form a feedback loop with differential regulations at different subcellular locations. We have developed FRET biosensors to monitor FAK activities at rafts vs. non-raft regions of plasma membrane in live cells. In response to cell adhesion on matrix proteins or stimulation by PDGF, the raft-targeting FAK biosensor showed a stronger FRET response than that at non-rafts. The FAK activation at rafts induced by PDGF is mediated by Src. In contrast, the FAK activation at rafts induced by adhesion is independent of Src activity, but rather is essential for Src activation. Thus, Src is upstream to FAK in response to chemical stimulation (PDGF), but FAK is upstream to Src in response to mechanical stimulation (adhesion). A novel biosensor has been developed to dynamically visualize the activity of membrane type-1-matrix metalloproteinase (MT1-MMP), which proteolytically remodels the extracellular matrix. Epidermal growth factor (EGF) directed active MT1-MMP to the leading edge of migrating live cancer cells with local accumulation of EGF receptor via a process dependent on an intact cytoskeletal network. In summary, FRET-based biosensors enable the elucidation of molecular processes and hierarchies underlying spatiotemporal regulation of biological and pathological processes, thus advancing our knowledge on how cells perceive mechanical/chemical cues in space and time to coordinate molecular/cellular functions.

Proceedings Article | 17 February 2009 Paper

Application of FRET biosensors and computational analysis for live cell imaging

Shaoying Lu, Yingxiao Wang

Proceedings Volume 7191, 719108 (2009) https://doi.org/10.1117/12.812183

KEYWORDS: Biosensors, Fluorescence resonance energy transfer, Diffusion, Biological research, Luminescence, Image registration, Automatic tracking, Image filtering, Live cell imaging, Visualization

Read Abstract +

Proceedings Article | 29 February 2008 Paper

The application of FRET biosensors to visualize Src activation

Yingxiao Wang, Shaoying Lu

Proceedings Volume 6868, 68680A (2008) https://doi.org/10.1117/12.764713

KEYWORDS: Biosensors, Fluorescence resonance energy transfer, Visualization, Luminescence, Proteins, Molecules, Optical filters, Wave propagation, Image filtering, Live cell imaging

Read Abstract +

Proceedings Volume Editor (1)

SPIE Conference Volume | 12 February 2009

Fluorescence In Vivo Imaging Based on Genetically Engineered Probes: From Living Cells to Whole Body Imaging IV

Conference Committee Involvement (1)

Fluorescence In Vivo Imaging Based on Genetically Engineered Probes: From Living Cells to Whole Body Imaging IV

25 January 2009 | San Jose, California, United States

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years