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Tingyin Wang

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Proceedings Article | 9 November 2010 Paper

A processing technology of two-photon microscopic image of nasopharyngeal cancer cells

Hongxin Lin, Tingyin Wang, Rong Chen, Yanan Wu, Shangyuan Feng, Zufang Huang, Jinping Lei, Lin Ou, Guannan Chen, Yongzeng Li

Proceedings Volume 7845, 784529 (2010) https://doi.org/10.1117/12.869806

KEYWORDS: Image segmentation, Image processing, Image enhancement, Edge detection, Image processing algorithms and systems, Cancer, MATLAB, Detection and tracking algorithms, Corrosion, Mathematical morphology

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Nasopharyngeal carcinoma (NPC) is one of the most common malignancies in china, with a deep and hidden localization. Recently, methods for early diagnosis of NPC has become one of the most important research topics in medical field. Early monitoring of morphological change of NPC cells during the carcinogenesis is of great importance, and early information extracted from the NPC cells during the initial stage of NPC is critical for diagnosis and treatment. In this paper, image processing methods for two-photon microscopic image of NPC cells was investigated with the purpose of providing useful information for early diagnosis and treatment of NPC. There is abundant information in a two-photon microscopic image of NPC cells, which can be analyzed and processed by means of computer and image pattern processing algorithm. In this paper, firstly, a mathematical method of transform of Bottom-hat based on Matlab platform was employed to enhance the image of NPC cells, making the image easier to distinguish; Then, several classical edge detection algorithms were compared and discussed, for example, Roberts operator, Prewitt operator, and Canny operator etc. According to the inherent characteristics of two-photon microscopic image of NPC cells, corrosion algorithm was used to define the edge of NPC cells. Furthermore, the article gets the iterative threshold segmentation after noise denoising, on the other hand, improved discriminant analysis was adopted for threshold segmentation of NPC cells, better results were obtained.

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