Dr. Tatevik Chalyan Profile

Dr. Tatevik Chalyan

SPIE Involvement:

Author | Career Lab Leader

Area of Expertise:

Laser Engineering , Silicon Photonics , Biosensors , Fluorescence Microscopy , Raman Spectroscopy , Microfluidics

Websites:

Company Website

Dr Tatevik Chalyan (07-01-1990) is a postdoctoral researcher at the Faculty of Engineering of the Vrije Universiteit Brussel (VUB). She works in the biophotonics group of B-PHOT Brussels Photonics. She mainly works in the field of Fluorescence Microscopy, Raman Spectroscopy, optofluidic design and validation.
She obtained her Master’s degree in Physics from the Yerevan State University, Armenia in 2012 and the degree of European Doctorate in Physics from the University of Trento, Italy in July 2018. Within the European Project SYMPHONY (Integrated SYsteM based on PHOtonic Microresonators and Microfluidic Components for rapid detectioN of toxins in milk and dairY products), she focused on developing an optical biosensor based on Integrated Silicon Photonics for rapid and precise detection of Aflatoxin M1 in milk and dairy products.
Between the Masters and PhD studies, 2011-2014, she worked at LT-PYRKAL cjsc. (Yerevan, Armenia) as a laser engineer. In the list of her duties were the assembly of solid-state Nd:YAG lasers for laser rangefinding system, as well as programming control and system alignment.
Tatevik has spent 3 months of her internship (04.2017-07.2017) in LioniX International B.V. working on the integrated silicon photonics-based biosensors.
Keywords: Optical instrumentation, measurement systems, spectroscopy, microscopy, lab-on-chip, biophotonics, integrated photonics.
Tatevik has authored and co-authored 8 peer-review journal papers and 5 conference proceedings, as well as co-authored 2 chapters in books. She was an editor of 2 conference proceedings. She was an invited speaker at SPIE Photonics Europe 2018 within the conference on Optical Sensing and Detection.

Proceedings Article | 23 May 2018 Presentation

Photonic biosensors for Fab'-AFM1 interaction study in real milk (Conference Presentation)

Tatevik Chalyan, Cristina Potrich, Erik Schreuder, Lorenzo Pavesi

Proceedings Volume 10680, 1068012 (2018) https://doi.org/10.1117/12.2307481

KEYWORDS: Biosensors, Biomedical optics, Mach-Zehnder interferometers, Fungi, Cancer, Liquids, Chromatography, Luminescence, Silicon photonics, Microrings

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Aspergillus is known as one of the most frequent toxigenic fungi in Europe. It produces aflatoxin B1 transformed into aflatoxin M1 (AFM1) present in milk. The International Agency for Research on Cancer (IARC) has included AFM1 in the group I human carcinogens. Acceptable maximum level of AFM1 in milk according to EU regulation is 50 ppt equivalent to 15.2×10-2 nM (the molecular weight of AFM1 is 328.27 g/mol). Up to now, the most used techniques for AFM1 detection in milk are time consuming Enzyme-Linked Immuno-Sorbent Assay (ELISA) and high-performance liquid chromatography associated to fluorescence (HPLC). Silicon photonics based biosensors such as Mach-Zehnder Interferometers and Microring Resonators thanks to their ability to be miniaturize and integrated with electronics and microfluidics in lab-on-a-chip devices, are new candidates to become faster, cheaper and more accurate tools for AFM1 detection in milk. Here, we validate photonic AFM1 biosensors based on an array of four Si3N4 asymmetric Mach-Zehnder Interferometers (aMZI) functionalized by F(ab’)2 fragments and passivated with casein. Analyses of AFM1 binding by Fab’ in MES buffer and in real milk samples, using different concentrations of AFM1, are performed. The dependence of the phase shifts to the AFM1 concentration allows to calculate the association and dissociation rate constants. The proposed biosensor is capable to detect AFM1 concentration down to 50 ppt. For the average dissociation constant (KD) of AFM1-Fab’ interaction, values of (1.8÷5)×10-8 M in MES buffer and 0.8×10-9 M in milk samples are measured. These are in the same order of magnitude as published results. The difference of one order of magnitude between KD in MES buffer and in milk might be caused by the fact that during the preparation of milk samples, an additional concentration of salt is added to the solution which yields a stronger ionic interaction to occur. Finally, the specificity of the interaction is confirmed by using blank solutions that are free from AFM1.

Proceedings Article | 29 August 2017 Presentation + Paper

Use of microring resonators for biospecific interaction analysis

Tatevik Chalyan, Geert A. J. Besselink, Rene Heideman, Lorenzo Pavesi

Proceedings Volume 10353, 103530Q (2017) https://doi.org/10.1117/12.2274132

KEYWORDS: Sensors, Vertical cavity surface emitting lasers, Biosensors, Proteins, Photonic devices, Biosensing, Integrated photonics, Microrings

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Proceedings Article | 29 April 2016 Paper

Biosensors based on Si3N4 asymmetric Mach-Zehnder interferometers

Tatevik Chalyan, Laura Pasquardini, Floris Falke, Manuela Zanetti, Romain Guider, Davide Gandolfi, Eric Schreuder, Cecilia Pederzolli, René Heideman, Lorenzo Pavesi

Proceedings Volume 9899, 98991S (2016) https://doi.org/10.1117/12.2227695

KEYWORDS: Sensors, Mach-Zehnder interferometers, Microfluidics, Vertical cavity surface emitting lasers, Biosensors, Photonic devices, Biosensing, Light sources, Single mode fibers, Silicon, Phase shifts, Refractive index, Phase measurement, Antimony, Liquids, Waveguides

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