Mr. Takashi Chishima Profile

Takashi Chishima

at Yokohama City Univ

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Proceedings Article | 2 July 1999 Paper

Green fluorescent protein: new light to visualize metastasis and angiogenesis in cancer

Meng Yang, Takashi Chishima, Eugene Baranov, Hiroshi Shimada, A. Moossa, Robert Hoffman

Proceedings Volume 3600, (1999) https://doi.org/10.1117/12.351020

KEYWORDS: Tumors, Green fluorescent protein, Visualization, Animal model studies, Cancer, Luminescence, Tissues, Lung, Lung cancer, Tumor growth modeling

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Green fluorescent protein (GFP)-expressing cell-lines have been established by our laboratory that permit the visualization and imaging of primary tumors and micrometastases in live tissue and live animals. Hamster and human cancer cell-lines were transfected with vectors containing the humanized GFP cDNA. Stable high-level expression of GFP was maintained in subcutaneously and orthotopically growing tumors in nude or SCID mice. Subsequent micro-metastases were visualized by GFP fluorescence in live tissue of systematic organs down to the single-cell level. GFP-expressing lung and prostate cancer were visualized to metastasize widely throughout the skeleton when implanted orthotopically in nude mice. With these GFP-cell lines, we have developed models that closely mimic the clinic situation. We have now developed a mean to visualize the onset and progression of angiogenesis of growing and spreading tumors by injecting a fluorescent rhodamine dye to the GFP-tumor-bearing mice indicate that the onset and extent of tumor angiogenesis depends on the site and type of tumor growing in the animal. These models are ideal for studying the mechanisms of cancer metastasis and for discovery of angiogenesis and metastasis inhibitors.

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