Sensitive detection of specific chemicals on site can be extremely powerful in many fields. Owing to its molecular fingerprinting capability, surface-enhanced Raman scattering has been one of the technological contenders. In this paper, we describe the novel use of DNA topological nanostructure on nanoporous gold disk array (NPGDA) chip for chemical sensing. NPGDA features large surface area and high-density plasmonic field enhancement known as “hot-spots”. Hence, NPGDA chip has found many applications in nanoplasmonic sensor development. This technique can provide novel label-free molecular sensing capability with high sensitivity and specificity. In this paper, we introduce a new concept of multimodal signal amplification by exploring the synergy of catalytic multiplication and plasmonic intensification.
Nanoplasmonic sensor has become a recent research focus due to its significant signal enhancement and robust signal transduction measured by various techniques. However, since the native gold surface does not have the capability to selectively bind target biomolecules, high molecular specificity has been a challenge. Nanoporous gold nanoparticle (NPG-NP) array chip showcases large specific surface area and high-density plasmonic field enhancement known as “hot-spots”. In this paper, we discuss strategies to enhance molecular specificity by functionalizing NPG-NP with unique bio-recognition elements towards both high sensitivity and specificity. A few examples will be given using existing and novel bio-recognition elements.
Sensitive detection of specific chemicals on site can be extremely powerful in many fields. Owing to its molecular fingerprinting capability, surface-enhanced Raman scattering has been one of the technological contenders. In this paper, we describe the novel use of DNA topological nanostructure on nanoporous gold nanoparticle (NPG-NP) array chip for chemical sensing. NPG-NP features large surface area and high-density plasmonic field enhancement known as “hotspots”. Hence, NPG-NP array chip has found many applications in nanoplasmonic sensor development. This technique can provide novel label-free molecular sensing capability and enables high sensitivity and specificity detection using a portable Raman spectrometer.
G-quadruplex, readily formed by the G-rich sequence, potentially distributes in over 40 % of all human genes, such as the telomeric DNA with the G-rich sequence found at the end of the chromosome. The G-quadruplex structure is supposed to possess a diverse set of critical functions in the mammalian genome for transcriptional regulation, DNA replication and genome stability. However, most of the currently available methods for G-quadruplex identification are restricted to fluorescence techniques susceptible to poor sensitivity. It is essential to propose methods with higher sensitivity to specifically recognize the G-quadruplexes. In this study, we demonstrate a label-free plasmonic biosensor for G-quadruplex detection by relying on the advantages of nanoporous gold (NPG) disks that provide high-density plasmonic hot spots, suitable for molecular recognition capability without the requirement for labeling processes.
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