Prof. Raimund J. Ober Profile

Prof. Raimund J. Ober

at University of Southampton

SPIE Involvement:

Conference Program Committee | Author

Publications (21)

Proceedings Article | 4 March 2019 Presentation

A system calibration protocol for widefield optical microscopy (Conference Presentation)

Sung Yong You, Sreevidhya Ramakrishnan, Jerry Chao, Anish Abraham, E. Sally Ward, Raimund Ober

Proceedings Volume 10883, 1088311 (2019) https://doi.org/10.1117/12.2510791

KEYWORDS: Calibration, Optical microscopy, Microscopy, Optical components, Optical filters, Imaging systems, Luminescence, Objectives, Error analysis, Image quality

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Proceedings Article | 14 March 2018 Presentation

Single molecule microscopy and resolution (Conference Presentation)

Raimund Ober

Proceedings Volume 10499, 104990O (2018) https://doi.org/10.1117/12.2314626

KEYWORDS: Molecules, Microscopy

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Proceedings Article | 14 March 2018 Presentation

A software framework for designing localization analyses and its use in optimizing single molecule localization (Conference Presentation)

Anish Abraham, Jerry Chao, Dukwhan Kim, Raimund Ober

Proceedings Volume 10499, 104990D (2018) https://doi.org/10.1117/12.2291020

KEYWORDS: Molecules, Software frameworks, Microscopy, Signal detection, Data analysis, Optical tracking, Visual analytics, Visualization, Interference (communication), Microscopes

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Proceedings Article | 17 February 2017 Paper

A state space based approach to localizing single molecules from multi-emitter images

Milad Vahid, Jerry Chao, E. Sally Ward, Raimund Ober

Proceedings Volume 10070, 100700J (2017) https://doi.org/10.1117/12.2253175

KEYWORDS: Biomedical engineering, Molecules, Microscopy, Super resolution microscopy, Image resolution, Optimization (mathematics), Computer simulations, Photon counting, Algorithm development, Reconstruction algorithms, Molecular photonics, Point spread functions, Detection and tracking algorithms, Super resolution

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Proceedings Article | 17 February 2017 Paper

Remote focusing multifocal plane microscopy for the imaging of 3D single molecule dynamics with cellular context

Jerry Chao, Ramraj Velmurugan, Sungyong You, Dongyoung Kim, E. Sally Ward, Raimund Ober

Proceedings Volume 10070, 100700L (2017) https://doi.org/10.1117/12.2251218

KEYWORDS: Molecules, Microscopy, Microscopes, Objectives, 3D image processing, Image analysis, Diffraction, Optical microscopy, 3D acquisition, Image acquisition, Source localization, Sensors, Luminescence, Data analysis

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Proceedings Article | 9 March 2016 Paper

Investigating the usage of point spread functions in point source and microsphere localization

Jerry Chao, Sripad Ram, E. Sally Ward, Raimund Ober

Proceedings Volume 9713, 97131M (2016) https://doi.org/10.1117/12.2208631

KEYWORDS: Super resolution microscopy, Optical microscopy, Estimation theory, Numerical integration, Point spread functions, Molecules, Image analysis, Microscopy, Data analysis, Error analysis, Photons, Data modeling, Statistical analysis, CCD image sensors, Interference (communication), Charge-coupled devices

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Proceedings Article | 26 August 2015 Paper

New results on the single molecule localization problem in two and three dimensions

Amir Tahmasbi, E. Sally Ward, Raimund Ober

Proceedings Volume 9554, 955402 (2015) https://doi.org/10.1117/12.2192008

KEYWORDS: Point spread functions, Molecules, Microscopy, 3D image processing, 3D modeling, Image analysis, 3D acquisition, Sensors, Interference (communication), Luminescence

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Proceedings Article | 9 March 2015 Paper

An information-theoretic approach to designing the plane spacing for multifocal plane microscopy

Amir Tahmasbi, Sripad Ram, Jerry Chao, Anish Abraham, E. Ward, Raimund Ober

Proceedings Volume 9330, 933011 (2015) https://doi.org/10.1117/12.2076769

KEYWORDS: Molecules, Microscopy, Photons, Particles, Sensors, 3D image processing, Luminescence, Software development, Manganese, Stereoscopy

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Proceedings Article | 22 February 2013 Paper

Two approximations for the geometric model of signal amplification in an electron-multiplying charge-coupled device detector

Jerry Chao, Sripad Ram, E. Sally Ward, Raimund Ober

Proceedings Volume 8589, 858905 (2013) https://doi.org/10.1117/12.2004621

KEYWORDS: Sensors, Electron multiplying charge coupled devices, Signal detection, Charge-coupled devices, Data modeling, Signal processing, Interference (communication), Stochastic processes, CCD image sensors, Molecules

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Proceedings Article | 3 February 2012 Paper

Localization accuracy in single molecule microscopy using electron-multiplying charge-coupled device cameras

Jerry Chao, E. Sally Ward, Raimund Ober

Proceedings Volume 8227, 82271P (2012) https://doi.org/10.1117/12.908951

KEYWORDS: Electron multiplying charge coupled devices, Charge-coupled devices, Molecules, Interference (communication), Signal processing, Photons, Data modeling, Cameras, Microscopy, Signal detection

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Proceedings Article | 24 February 2010 Paper

Comparison of estimation algorithms in single-molecule localization

Anish Abraham, Sripad Ram, Jerry Chao, E. Sally Ward, Raimund Ober

Proceedings Volume 7570, 757004 (2010) https://doi.org/10.1117/12.842178

KEYWORDS: Molecules, Data modeling, Performance modeling, Stochastic processes, Software development, Microscopy, Error analysis, Nonlinear optics, Super resolution, Cameras

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Proceedings Article | 18 February 2010 Paper

Three-dimensional single molecule tracking of quantum-dot labeled antibody molecules using multifocal plane microscopy

Sripad Ram, Prashant Prabhat, Jerry Chao, E. Sally Ward, Raimund Ober

Proceedings Volume 7575, 75750J (2010) https://doi.org/10.1117/12.848940

KEYWORDS: Molecules, 3D image processing, Microscopy, Super resolution, Stereoscopy, Microscopes, Plasma, Particles, Live cell imaging, Image resolution

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Proceedings Article | 4 March 2009 Paper

Dual objective fluorescence microscopy for single molecule imaging applications

Sripad Ram, Prashant Prabhat, E. Sally Ward, Raimund Ober

Proceedings Volume 7184, 71840C (2009) https://doi.org/10.1117/12.808259

KEYWORDS: Objectives, Microscopes, Molecules, Photons, Nanoprobes, Luminescence, Lenses, Microscopy, Cameras, Data acquisition

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Proceedings Article | 21 February 2008 Paper

Overcoming the depth discrimination barrier in widefield microscopes: 3D single molecule tracking with high axial accuracy

Sripad Ram, Jerry Chao, Prashant Prabhat, E. Sally Ward, Raimund Ober

Proceedings Volume 6862, 68620O (2008) https://doi.org/10.1117/12.763097

KEYWORDS: Molecules, Microscopes, Microscopy, 3D image processing, Plasma, Sensors, Photodetectors, 3D modeling, Data acquisition, Objectives

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Proceedings Article | 12 February 2008 Paper

Resolution in 3D in multifocal plane microscopy

Jerry Chao, Sripad Ram, Anish Abraham, E. Sally Ward, Raimund Ober

Proceedings Volume 6861, 68610Q (2008) https://doi.org/10.1117/12.763084

KEYWORDS: Molecules, 3D metrology, 3D image processing, Image resolution, Sensors, Microscopes, Photodetectors, Microscopy, Image processing, Image segmentation

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Proceedings Article | 14 February 2007 Paper

A novel approach to determining the three-dimensional location of microscopic objects with applications to 3D particle tracking

Sripad Ram, Jerry Chao, Prashant Prabhat, E. Sally Ward, Raimund Ober

Proceedings Volume 6443, 64430D (2007) https://doi.org/10.1117/12.698763

KEYWORDS: Molecules, Microscopes, 3D image processing, Sensors, Image processing, Photons, Data acquisition, 3D modeling, Microscopy, Objectives

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Proceedings Article | 14 February 2007 Paper

A novel 3D resolution measure for optical microscopes with applications to single molecule imaging

Sripad Ram, Anish Abraham, E. Sally Ward, Raimund Ober

Proceedings Volume 6444, 64440D (2007) https://doi.org/10.1117/12.698765

KEYWORDS: Molecules, 3D metrology, Sensors, Image resolution, Optical microscopes, Photodetectors, 3D image processing, Optical resolution, Photon counting, Data acquisition

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Proceedings Article | 23 February 2006 Paper

A novel stochastic resolution criterion for fluorescence microscopes

Sripad Ram, E. Sally Ward, Raimund Ober

Proceedings Volume 6090, 60900J (2006) https://doi.org/10.1117/12.644360

KEYWORDS: Photons, Molecules, Image resolution, Stochastic processes, Microscopes, Sensors, Luminescence, Data acquisition, Microscopy, Objectives

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Proceedings Article | 23 February 2006 Paper

Simultaneous imaging of several focal planes in fluorescence microscopy for the study of cellular dynamics in 3D

Prashant Prabhat, Sripad Ram, E. Sally Ward, Raimund Ober

Proceedings Volume 6090, 60900L (2006) https://doi.org/10.1117/12.644343

KEYWORDS: Cameras, Microscopes, Microscopy, Luminescence, 3D image processing, Proteins, Sensors, Calibration, Data acquisition, Objectives

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Proceedings Article | 29 March 2005 Paper

How accurately can a single molecule be localized in three dimensions using a fluorescence microscope?

Sripad Ram, E. Sally Ward, Raimund Ober

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.587878

KEYWORDS: Molecules, Sensors, Microscopes, Luminescence, Photodetectors, Data acquisition, Data modeling, 3D modeling, Stochastic processes, Microscopy

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Proceedings Article | 24 March 2005 Paper

Three-dimensional state space realization algorithm: noise suppression of fluorescence microscopy images and point spread functions

Xuming Lai, E. Sally Ward, Zhiping Lin, Raimund Ober

Proceedings Volume 5701, (2005) https://doi.org/10.1117/12.587051

KEYWORDS: Point spread functions, 3D image processing, Microscopy, Deconvolution, Luminescence, Algorithm development, Computer simulations, Reconstruction algorithms, Algorithms, 3D image restoration

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Showing 5 of 21 publications

Conference Committee Involvement (19)

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXII

25 January 2025 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI

31 January 2024 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX

1 February 2023 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX

26 January 2022 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII

6 March 2021 | Online Only, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII

4 February 2020 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI

5 February 2019 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV

29 January 2018 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV

30 January 2017 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII

15 February 2016 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII

10 February 2015 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI

3 February 2014 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX

5 February 2013 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX

24 January 2012 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII

25 January 2011 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII

26 January 2010 | San Francisco, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI

27 January 2009 | San Jose, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV

21 January 2008 | San Jose, California, United States

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV

24 January 2007 | San Jose, California, United States

Showing 5 of 19 Conference Committees

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