Prof. António Miguel Morgado Profile

Prof. António Miguel Morgado

Professor at Univ de Coimbra

SPIE Involvement:

Author

Area of Expertise:

Optical Imaging , Optoelectronic Instrumentation , Multiphoton Microscopy , Optical Coherence Elastography , FLIM

Websites:

Personal Website | Company Website

Publications (15)

Proceedings Article | 15 March 2023 Presentation

Quantitative analysis of collagen organization from second-harmonic generation images (Conference Presentation)

Pedro Guimarães, Maria Mateus, António Miguel Morgado, Ana Batista

Proceedings Volume PC12384, PC1238407 (2023) https://doi.org/10.1117/12.2645686

KEYWORDS: Collagen, Second-harmonic generation, Quantitative analysis, Fourier transforms, Tissues, Network security, Data analysis

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Proceedings Article | 15 March 2023 Presentation + Paper

Evaluation of skin care products using optical coherence elastography

Rita Bernardo, José Domingues, Rui Bernardes, Miguel Morgado, Ana Batista

Proceedings Volume 12381, 123810A (2023) https://doi.org/10.1117/12.2648348

KEYWORDS: Skin, Elasticity, Optical coherence tomography, Tissues, Elastography, Optical coherence, Wave propagation, Materials properties, Biomechanics, Actuators

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Proceedings Article | 18 April 2021 Poster + Presentation + Paper

Compressive single pixel phosphorescence lifetime and intensity simultaneous imaging: a pilot study using oxygen sensitive biomarkers

Patrícia Santos, Pedro Vaz, Andreia Sofia Gaudêncio, Miguel Morgado, Nelson A. Pereira, Marta Pineiro, T.M.V.D. Pinho e Melo, João Cardoso

Proceedings Volume 11775, 1177511 (2021) https://doi.org/10.1117/12.2588266

KEYWORDS: Oxygen, Phosphorescence, Image compression, Sensors, Image quality, Reconstruction algorithms, Quenching (fluorescence), Photodiodes, Modulators, Electronics

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Compressive single pixel imaging (C-SPI) is a novel imaging technique able to reconstruct images using only a single pixel detector and a partial measurement of the scene. This technique uses different structured illumination, generated by a spatial modulator, to illuminate the scene and measures the reflected or transmitted light intensities. This compressive stage allows for a sub-Nyquist set of measurements, which significantly increases the technique efficiency, while maintaining a good reconstruction image quality. An experimental setup was developed to study the ability of C-SPI to determine the lifetime and mean intensity of an oxygen sensitive biomarker (Pt(II) ring-fused chlorins). This biomarker phosphorescence is quenched in the presence of oxygen resulting in smaller intensity and lifetime. A structured illumination system was used to stimulate the sample with Hadamard patterns, in the range between 428 nm and 620 nm, while a photodiode collected the emitted light peaking at 756nm. The imaged scene was composed of two toluene solutions with the same biomarker concentration (1 μM), one in the presence of oxygen and the other in its absence. The images were reconstructed using total variation minimization by augmented lagrangian and alternating direction algorithms (TVAL3) with compressive ratios of 25%, 10% and 5%. The proposed method was able to spatially locate the sample contained in a deoxygenated environment in two distinct spatial locations. The deoxygenated sample lifetime and mean intensity of the reconstructed images were, respectively, approximate 20 μs and 2.1×10^-3 AU, for the first location, and 20 μs and 4.0×10^-3 AU for the second location. Regarding the oxygenated sample, the results were scattered in a long range for lifetime but achieved a mean intensity of approximately 4.8×10^-5 AU. Consistent results were obtained for the three compression ratios without major loss in image quality. The proposed method showed that SPI has the ability to perform simultaneous phosphorescence lifetime and intensity imaging. The introduction of compressive sensing makes this technology more attractive to practical applications because it lowers the amount of time necessary to image the sample. The C-SPI is a simple and less expensive technique because it dismisses the use of a fast two dimensional detector (CCD) and the associated electronics, as well as mechanical scanning procedures. Also, multiple single pixel detectors, sensitive to different wavelengths, can make these instruments versatile and allow for simultaneous phosphorescence biomarkers analysis. The next steps in the research will be to study changes in the sample concentration and different percentages of dissolved oxygen.

Proceedings Article | 14 March 2016 Paper

Time-gated FLIM microscope for corneal metabolic imaging

Susana Silva, Ana Batista, José Paulo Domingues, Maria João Quadrado, António Miguel Morgado

Proceedings Volume 9712, 97122C (2016) https://doi.org/10.1117/12.2214182

KEYWORDS: Microscopes, Cornea, Fluorescence lifetime imaging, Image intensifiers, CCD cameras, Objectives, Digital micromirror devices, Reflectivity

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SPIE Journal Paper | 8 March 2016 Open Access

Two-photon spectral fluorescence lifetime and second-harmonic generation imaging of the porcine cornea with a 12-femtosecond laser microscope

Ana Batista, Hans Georg Breunig, Aisada Uchugonova, António Miguel Morgado, Karsten König

JBO, Vol. 21, Issue 03, 036002, (March 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.3.036002

KEYWORDS: Second-harmonic generation, Cornea, Signal detection, Collagen, Luminescence, Fluorescence lifetime imaging, Photons, Microscopes, Spectral resolution, Sensors

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Proceedings Article | 15 July 2015 Paper

Development of a time-gated fluorescence lifetime microscope for in vivo corneal metabolic imaging

Susana Silva, Ana Batista, Olga Castejón, Maria João Quadrado, José Paulo Domingues, Miguel Morgado

Proceedings Volume 9537, 953709 (2015) https://doi.org/10.1117/12.2183945

KEYWORDS: Luminescence, Microscopes, Fluorescence lifetime imaging, In vivo imaging, Objectives, Picosecond phenomena, Cornea, Digital micromirror devices, Mirrors, Mode conditioning cables

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Proceedings Article | 20 March 2015 Paper

Two-photon autofluorescence lifetime and SHG imaging of healthy and diseased human corneas

Ana Batista, Hans Georg Breunig, Aisada Uchugonova, Berthold Seitz, António Miguel Morgado, Karsten König

Proceedings Volume 9307, 93071Q (2015) https://doi.org/10.1117/12.2077569

KEYWORDS: Cornea, Second-harmonic generation, Pathology, Luminescence, Fluorescence lifetime imaging, Collagen, Signal detection, Microscopes, Pulsed laser operation, Sensors

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Proceedings Article | 5 March 2015 Paper

Characterization of porcine eyes based on autofluorescence lifetime imaging

Ana Batista, Hans Georg Breunig, Aisada Uchugonova, António Miguel Morgado, Karsten König

Proceedings Volume 9329, 93290E (2015) https://doi.org/10.1117/12.2077563

KEYWORDS: Retina, Cornea, Eye, Fluorescence lifetime imaging, Second-harmonic generation, Luminescence, Crystals, Signal detection, Collagen, Spectroscopy

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SPIE Journal Paper | 8 September 2014 Open Access

Low-level laser therapy on skeletal muscle inflammation: evaluation of irradiation parameters

Matias Mantineo, João Páscoa Pinheiro, António Miguel Morgado

JBO, Vol. 19, Issue 09, 098002, (September 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.9.098002

KEYWORDS: Continuous wave operation, Inflammation, Laser therapeutics, Tissues, Blood, Skin, Tissue optics, Injuries, Thermal effects, Absorption

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Proceedings Article | 28 February 2014 Paper

Label-free SHG imaging and spectral FLIM of corneas using a sub-15 fs laser microscope

Ana Batista, Hans Georg Breunig, Aisada Uchugonova, Berthold Seitz, António Miguel Morgado, Karsten König

Proceedings Volume 8930, 89300V (2014) https://doi.org/10.1117/12.2037640

KEYWORDS: Cornea, Second-harmonic generation, Fluorescence lifetime imaging, Collagen, Signal detection, Luminescence, Femtosecond phenomena, Microscopes, Multiphoton microscopy, Sensors

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Proceedings Article | 28 February 2014 Paper

Detection of back-reflected SHG from corneal histological sections

Ana Batista, António Miguel Morgado, Karsten König

Proceedings Volume 8948, 89482B (2014) https://doi.org/10.1117/12.2038251

KEYWORDS: Signal detection, Second-harmonic generation, Reflectivity, Dielectric mirrors, Mirrors, Silver, Aluminum, Luminescence, Photons, Microscopes

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Proceedings Article | 18 February 2014 Paper

Evaluation of low level laser therapy irradiation parameters on rat muscle inflammation through systemic blood cytokines

Matias Mantineo, João Pinheiro, António Morgado

Proceedings Volume 8932, 89320M (2014) https://doi.org/10.1117/12.2039342

KEYWORDS: Inflammation, Blood, Laser therapeutics, Tissues, Injuries, Semiconductor lasers, Absorption, Oxygen, Satellites

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Proceedings Article | 18 November 2013 Paper

Methodology for assessment of low level laser therapy (LLLT) irradiation parameters in muscle inflammation treatment

M. Mantineo, J. Pinheiro, A. Morgado

Proceedings Volume 9032, 90320P (2013) https://doi.org/10.1117/12.2044689

KEYWORDS: Inflammation, Laser therapeutics, Blood, Tissues, Statistical analysis, Medicine, Molecular mechanisms, Injuries, Oxygen, Semiconductor lasers

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Proceedings Article | 18 November 2013 Paper

Low level laser therapy on injured rat muscle: assessment of irradiation parameters

M. Mantineo, J. Pinheiro, A. Morgado

Proceedings Volume 8785, 8785DU (2013) https://doi.org/10.1117/12.2021689

KEYWORDS: Inflammation, Laser therapeutics, Light emitting diodes, Blood, Radiation effects, Beam controllers, Laser tissue interaction, Medicine, Molecular mechanisms, Semiconductor lasers

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Proceedings Article | 24 June 2013 Paper

Low level laser therapy on injured rat muscle

M. Mantineo, J. Pinheiro, A. Morgado

Proceedings Volume 8803, 88030E (2013) https://doi.org/10.1117/12.2032496

KEYWORDS: Laser therapeutics, Inflammation, Blood, Tissues, Molecular mechanisms, Oxygen, Medicine, Near infrared, Injuries, Semiconductor lasers

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Showing 5 of 15 publications

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