Quantum dots promise to revolutionize the way fluorescence imaging is used in the Cell Biology field. The unique fluorescent spectral characteristics, high photostability, low photobleaching and tight emission spectra of quantum dots, position them above traditional dyes. Here we will address the ability of EviTags, which are water stabilized quantum dot products from Evident Technologies, to behave as effective FRET donors in cells. EviTag-Hops Yellow (HY; Emission 566nm; Donor) conjugated to biotin were bound to stretapvidin-Alexa568 (Acceptor) conjugates. These HYbiotin-streptavidin-Alexa568 FRET EviTag conjugates were then internalized by fluid-phase into non-polarized MDCK cells. Confocal microscopy detects these FRET EviTag conjugates in endocytic compartments, suggesting that EviTags can be used to track fluid-phase internalization and trafficking. EviTags are shown here to be effective FRET donors when internalized into cells. Upon pairing with the appropriate acceptor dyes, quantum dots will reduce the laborious data processing that is required to compensate for bleed through contamination between organic dye donor and acceptor pair signals. The EviTag technology will simplify and expand the use of FRET in the analysis of cellular processes that may involve protein-protein interactions and other complex cellular processes.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.