Liv Furuberg, Michal Mielnik, Ib-Rune Johansen, Jörg Voitel, Anja Gulliksen, Lars Solli, Frank Karlsen, Tobias Bayer, Friedhelm Schönfeld, Klaus Drese, Helen Keegan, Cara Martin, John O'Leary, Lutz Riegger, Peter Koltay
The aim of the MicroActive project is to develop an instrument for molecular diagnostics. The instrument will first be
tested for patient screening for a group of viruses causing cervical cancer. Two disposable polymer chips with reagents
stored on-chip will be inserted into the instrument for each patient sample. The first chip performs sample preparation of
the epithelial cervical cells while mRNA amplification and fluorescent detection takes place in the second chip. More
than 10 different virus markers will be analysed in one chip. We report results on sub-functions of the amplification chip.
The sample is split into smaller droplets, and the droplets move in parallel channels containing different dried reagents
for the different analyses. We report experimental results on parallel droplet movement control using one external pump
only, combined with hydrophobic valves. Valve burst pressures are controlled by geometry. We show droplet control
using valves with burst pressures between 800 and 4500 Pa. We also monitored the re-hydration times for two necessary
dried reagents. After sample insertion, uniform concentration of the reagents in the droplet was reached after respectively
60 s and 10 min. These times are acceptable for successful amplification. Finally we have shown positive amplification
of HPV type 16 using dried enzymes stored in micro chambers.
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