The detection of bacterial deoxyribonucleic acid (DNA) can be helpful to quickly identify a bacterium. A rapid SERS detection of bacterial DNA using microwave synthesized silver (Ag) nanoparticles (NPs) was reported. The microwave synthesizer technique offers quick synthesis of Ag NPs providing control pressure and uniform heat. The NPs based SERS can detect the genomic content of Escherichia coli (E. coli) NC_000913.3 and Pseudomonas aeruginosa (P. aeruginosa) NZ_CP012001 up to 50 ng/μL and 40 ng/μL respectively.
We have investigated the enhanced Raman spectra of AMR bacteria strains of E. coli using silver coated silicon nanowires SERS assay. Three different E. coli strains, E. coli CCUG17620, NCTC 13441, and A239, were detected using two different excitation laser wavelengths. We found stable and enhanced SERS spectrum using 785 nm laser as opposed to 532 nm. Future development of SERS-chip could offer a reliable platform for direct identification of the pathogen in bio-fluid samples at strains level.
We fabricated SERS-based biosensors using the GLAD technique. The enhancement in Raman signal was investigated using 0.01M Rhodamine 6G solution, and it was employed to detect and identify biological pathogens like E-Coli bacteria.
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