Biomarker-specific photothermal nanoparticles that can efficiently sense markers that are overexpressed in distinguished adenocarcinomas have attracted much interest in an aspect of efficacy increase of cancer treatment. We demonstrated a promising prospect of a smart photothermal therapy agent employing anti-epidermal growth factor receptor aptamer (Apt EGFR )-conjugated polyethylene glycol (PEG) layted gold nanorods (Apt EGFR -PGNRs). The cetyltrimethylammonium bromide bilayer on GNRs was replaced with heterobifunctional PEG (COOH-PEG-SH) not only to serve as a biocompatible stabilizer and but also to conjugate Apt EGFR . Subsequently, to direct photothermal therapy agent toward epithelial cancer cells, the carboxylated PEGylated GNRs (PGNRs) were further functionalized with Apt EGFR using carbodiimide chemistry. Then, to assess the potential as biomarker-specific photothermal therapy agent of synthesized Apt EGFR -PGNRs, the optical properties, biocompatibility, colloidal stability, binding affinity, and epicellial cancer cell killing efficacy in vitro/in vivo under near-infrared laser irradiation were investigated. As a result, Apt EGFR -PGNRs exhibit excellent tumor targeting ability and feasibility of effective photothermal ablation cancer therapy.
Biomarker-specific photothermal nanoparticles that can efficiently sense the markers, which are overexpressed in
distinguished adenocarcinomas, has attracted much interest in an aspect of efficacy increase of cancer treatment. In this
study, we demonstrated a promising prospect of smart photothermal therapy agent employing anti-epidermal growth
factor receptor aptamer (AptEGFR)-conjugated polyethylene glycol (PEG)layted gold nanorods (AptEGFR-PGNRs). The
cetyltrimethylammonium bromide bilayer on GNRs was replaced with heterobifunctional polyethylene glycol (COOHPEG-
SH) not only to serve as a biocompatible stabilizer and but also to conjugate AptEGFR. Subsequently, to direct
photothermal therapy agent toward epithelial cancer cells, the carboxylated PEGylated GNRs (PGNRs) were further
functionalized with AptEGFR using carbodiimide chemistry. And then, to assess the potential as biomarker-specific
photothermal therapy agent of synthesized AptEGFR-PGNRs, the optical properties, biocompatibility, colloidal stability,
binding affinity and epicellial cancer cell killing efficacy in vitro/in vivo under NIR laser irradiation were investigated.
As a results, AptEGFR-PGNRs exhibit excellent tumor targeting ability and feasibility of effective photothermal ablation
cancer therapy.
KEYWORDS: Nanoparticles, Magnetism, Cancer, Magnetic resonance imaging, Near infrared, Tumors, Scanning electron microscopy, Oncology, Diagnostics, Transmission electron microscopy
Hybrid organic-inorganic near-infrared responsive magnetic nanoparticles were synthesized for theragnosis combined with localized therapy. In detail, inorganic super-paramagnetic nanoparticles were embedded inside organic polyaniline matrix, which enables localized photothermal therapy upon NIR illumination under intracellular acidic/oxidative condition. In this structure, super-paramagnetic nanoparticle works as MRI contrast agent, that enables the visualization of a tumor and polyaniline works for near-infrared responsive tumor ablation.
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