We proposed here a new method of encoding and extracting the 3D position of fluorescent micro particles. Cross-correlation matching method is employed to localize the axial position of fluorescent microsphere. And the localization accuracy of the proposal is compared with that of the traditional diffraction reconstruction algorithm. The effectiveness of the method is verified by both simulations and experiments, and the localization accuracy of the method are evaluated quantitatively. This method overcomes the limitation of the imaging depth in the traditional single molecule localization, reduces the influence of optical aberration on the localization accuracy, and it possess the potential of achieving the isotropic super resolution localization of the sample.
Fresnel incoherent correlation holography as a self-interference holographic technique is employed as three dimensional (3D) localization method of fluorescent microspheres, thus to obtain 3D microscopic imaging. In order to promote the localization accuracy and the reconstructed image quality, autofocus algorithm is introduced to help finding accurate reconstruction distance in the Fresnel Incoherent Correlation Holographic (FINCH) localization microscopic imaging. The performances of autofocus algorithm under different evaluation parameters of reconstruction images are investigated, and the suitable autofocus algorithm are used during hologram reconstruction. The localization algorithm is utilized based on the reconstructed image. The localization accuracy and imaging quality are compared with and without employing the autofocus algorithm. The enhanced localization accuracy is realized in the actual FINCH localization microscopic imaging system.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.