In this study, surgical operation for the myocardial ischemia of rat was used to simulate the model of critically ill patients. Speckle variance optical coherence tomography was applied to study the effects of myocardial ischemia on blood microcirculation. The main method is to simulate the severe condition through the heart ligation operation in rats, and then the OCT system is used to image the ear of rats. First of all, the variance operation is performed on the same position of the structure map through speckle variance algorithm, so as to obtain the blood flow intensity information. Finally, the vascular morphological structure can be roughly seen through the basic vascular enhancement algorithm. A comparison of vascular images before and after heart ligation surgery revealed that surgery had a significant effect on the circulation of microvessels, and many microvessels were lost intermittently after surgery. Accurate in vivo study model is very important to obtain OCT images and accurate analysis, and it is of great value to promote the development of microangiography4 and its clinical application.
To explore the damage range in the photothermal treatment at different temperatures, a temperature-feedback photothermal control system was developed. The system used an infrared thermal imager to noninvasively monitor the temperature .so it could avoid the damage caused by thermocouple measurement and apply the PID controller to achieve the desired temperature(1). the range of damage at the surface and the depth of internal damage were recorded at the different temperatures, which are based on the temperature-feedback photothermal control system. Finally, the recorded data are used to fit the curve by linear regression, and the damage depth was predicted according to the range of external damage at the surface. The technique could be a potential application for monitoring tumor treatment.
Photothermal therapy of tumors has become an important method. In recent years, the method has been widely studied in tumor therapy, and the corresponding results has been obtained well. However, it is still not been solved the effects of the heat on the tumor and its surrounding tissues, and the temperature control in corresponding tissues during the treatment process. In our study, the mouse skin was chosen as the research object. Infrared thermal imager and optical coherence tomography (OCT) were combined to monitor the photothermal therapy in real time in vivo. Temperature and morphological structure were obtained during the photothermal therapy process. The results will provide effective guidance for the photothermal therapy of tissue.
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