Mr. James J. Pasquesi Profile

James J. Pasquesi

Physicist at Northrop Grumman Systems Corp

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Proceedings Article | 20 February 2006 Paper

Detection of ultrastructural changes in genetically altered and exercised skeletal muscle using PS-OCT

James Pasquesi, Simon Schlachter, Marni Boppart, Eric Chaney, Stephen Kaufman, Stephen Boppart

Proceedings Volume 6079, 607926 (2006) https://doi.org/10.1117/12.646584

KEYWORDS: Birefringence, Polarization, Tissues, Optical fibers, Modulators, Proteins, Control systems, Luminescence, Electrodes, Optical coherence tomography

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Birefringence of skeletal muscle has been associated with the ultrastructure of individual sarcomeres, specifically the arrangement of A-bands corresponding to the thick myosin filaments. Murine skeletal muscle (gastrocnemius) was imaged with a fiber-based PS-OCT imaging system to determine the level of birefringence present in the tissue under various conditions. In addition to muscle controls from wild-type mice, muscle from abnormal mice included: genetically-modified (mdx) mice which model human muscular dystrophy, transgenic mice exhibiting an overexpression of integrin (α7β1), and transgenic integrin (α7β1)knockout mice. Comparisons were also made between rested and exercised muscles to determine the effects of exercise on muscle birefringence for each of these normal and abnormal conditions. The PS-OCT images revealed that the presence of birefringence was similar in the rested muscle with dystrophy-like features (i.e., lacking the structural protein dystrophin - mdx) and in the integrin (α7β1)knockout muscle when compared to the normal (wild-type) control. However, exercising these abnormal muscle tissues drastically reduced the presence of birefringence detected by the PS-OCT system. The muscle exhibiting an overexpression of integrin (α7β1) remained heavily birefringent before and after exercise, similar to the normal (wild-type) muscle. These results suggest that there is a distinct relationship between the degree of birefringence detected using PS-OCT and the sarcomeric ultrastructure present within skeletal muscle.

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