We have developed novel techniques of protein crystallization and processing using femtosecond laser, all solid-state
193 nm laser, and solution stirring. Femtosecond laser technique enables us to extremely increase the crystallization
probability, and to trigger the nucleation at low supersaturation of solution where spontaneous nucleation dose not occur.
Since the laser-induced nuclei grow slowly in the low-range supersaturated solution, the crystals exhibit high
crystallinity. The processing techniques using femtosecond laser and all solid-state 193 nm laser are effective for
processing and manipulation of protein crystals without significant damage. Solution stirring technique also contributes
the improvement of the crystal quality. We have succeeded in obtaining high-quality crystals of various proteins using
the techniques, and revealing the precise structure of the protein molecules from the X-ray analysis. These techniques
can accelerate structural biology and subsequent structure-based drug discoveries, resulting in important revelations in
these fields.
For practical holographic video system, it is important to generated holographic fringe as fast as possible. We have proposed an approximation method that can calculate the Fresnel hologram fast. With the proposed method, a hologram with more than one million pixels can be calculated less than one second with a personal computer. To compute the hologram, an object is assumed as a collection of self-illuminated points and the fringes from each object point are superposed. To determine the fringe, a distance between object point and sampling point on the hologram is used to obtain phase of the light. Since sampled hologram usually has small pixel intervals, the difference of the distance values between adjacent pixels is also small and its second order difference becomes a constant. Therefore, the distance value at certain pixel can be obtained from the neighbor pixel with simple additions. The experimental results show that the proposed method is quite effective for the holographic video display with full parallax.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.