Dr. H. Edward Ayliffe Profile

Dr. H. Edward Ayliffe

at ORFLO Technologies

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Proceedings Article | 26 March 1998 Paper

Microfabricated electric impedance chamber for the electrical characterization of single cells

H. Edward Ayliffe, Richard Rabbitt, A. Bruno Frazier

Proceedings Volume 3258, (1998) https://doi.org/10.1117/12.304366

KEYWORDS: Electrodes, Electroluminescence, Gold, Glasses, Data modeling, Dielectrics, Capacitance, Dielectric polarization, Microfluidics, Semiconducting wafers

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Micromachining technologies were applied to fabricate metal- electrode-instrumented microchannels with cross-sectional dimensions similar in size to blood cells. The instruments enable electric impedance measurement of femptoliter quantities of materials and solutions. The completed micro- electric impedance devices were characterized with varying concentrations of phosphate buffered saline solutions, DI water, and air in the recording zone. With the microdevices on the stage of an inverted light microscope, individual living cells were positioned tightly between metal electrodes using mechanical suction. Impedance spectra form 100 Hz to 2 MHz measured in isolated toadfish red blood cells (RBCs) and human neutrophils were distinct and demonstrated the ability to permeate the cell membrane at high frequency. The cell/shunt path cut-off frequency were approximately 400 kHz at -3dB indicating that non- invasive electric impedance characterization of the cytoplasm may be feasible for his configuration. In addition, the area specific membrane capacitance was estimated for both cell types by fitting the data to a simple RC circuit model.

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