KEYWORDS: Acquisition tracking and pointing, In vivo imaging, Tissues, Photoacoustic tomography, Imaging systems, Data acquisition, Computing systems, Ultrasonography, Transducers, Signal processing
Background: Photoacoustic tomography (PAT) is an evolving technique that is capable of obtaining real-time, high-resolution images of tissues. The purpose of this research was to evaluate morphological and functional information of the rabbit airway using PAT. Methods: PAT experiments were performed with nine New Zealand white rabbits. Here we measured the cross-sectional tracheal wall thickness and cross-sectional area of airway in vivo and in vitro. PAT airway images and the corresponding histological findings were compared. Results: PAT images showed the continuous variation of tracheal lumen size at different phases of the respiratory cycle. There were no significant differences between tracheal wall thickness by PAT imaging in vivo and the corresponding pathological tissue (p>0.05). No significant differences were found between cross-sectional area of airway wall in vivo and its corresponding fresh specimens by PAT imaging (p<0.05), while differences were found between cross-sectional area of airway wall in vivo and its corresponding formalin-fixed specimen by PAT imaging (p<0.01).
Rheumatoid arthritis (RA) is the leading cause of disability among people over 50-year-old. However, the diagnosis of RA is a complex problem because of the complexity of symptoms and structures. While current clinical imaging techniques play an important role in the diagnosis of RA, they do have several limitations. As an emerging hybrid modality, thermoacoustic tomography (TAT) provides high electromagnetic contrast and high ultrasonic resolution. Our early work showed that TAT could provide high quality anatomic structures of peripheral joints in healthy human fingers. In this study, we applied TAT to the detection of RA in the finger joint. Ultrasound imaging (US) was performed to verify TAT findings. The TAT images of the RA joint displayed abnormalities compared to the healthy ones, and they matched well with the US images. The initial results demonstrate that TAT has the potential to be conducive to detection of RA in vivo, which also provide a foundation for its clinical application to the diagnosis of RA.
KEYWORDS: Brain, Neuroimaging, Hemodynamics, Acquisition tracking and pointing, Photoacoustic tomography, Photoacoustic spectroscopy, In vivo imaging, Brain mapping, Visualization, Imaging systems
While medical imaging is playing an increasingly important role in understanding and clarifying acupuncture theory in general, it is still challenging to systematically understand the operational mechanisms of acupuncture on the central and peripheral nerve systems using the existing acupuncture imaging tools. Here, we report an application of photoacoustic tomography (PAT) to mapping cerebral hemodynamics response to acupuncture in mice. Huantiao acupoint (GB 30) is selected as the stimulating points in this study. In vivo photoacoustic images of cerebral hemodynamics response to acupuncture are obtained in ten functional regions of a mouse brain. Real-time photoacoustic signal changes in brain cortex and dynamic changes in total hemoglobin (HbT) concentration are tracked at 532 nm. Our results suggest that PAT provides imaging basis on cerebral hemodynamic response to acupuncture, and may have great potential for understanding the operational mechanisms of acupuncture on the central nerve systems.
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