Ms. Cara A. Chlebicki Profile

Cara A. Chlebicki

at Beckman Laser Institute and Medical Clinic

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 8 February 2008 Paper

Multiphoton microscopy imaging of collagen fiber layers and orientation in the tympanic membrane

R. Jackson, C. Chlebicki, T. Krasieva, S. Puria

Proceedings Volume 6842, 68421D (2008) https://doi.org/10.1117/12.774353

KEYWORDS: Collagen, Second-harmonic generation, Multiphoton microscopy, Harmonic generation, Luminescence, Bone, Tissues, Molecules, Microscopy, Image quality

Read Abstract +

Greater understanding of tympanic membrane (TM) biomechanics has the potential to guide future advances in medical technology related to its surgical repair (myringoplasty). The pars tensa of the TM is a composite structure with two collagen fiber layers that provide the main scaffolding for the TM. The external layer is arranged in an approximately radial configuration, and the other is arranged in an approximately circumferential configuration. A more detailed knowledge of collagen fiber orientation and volume fraction could greatly improve existing mechanical simulations of the TM. To address this, we employed multiphoton microscopy (MPM) imaging of the TM in two modalities: second harmonic generation (SHG) and two-photon fluorescence (TPF). The unique spectral signature of SHG allows selective imaging of collagen fibers. TPF also produces images of fibrillar-type collagen but lacks the specificity of SHG. Both the SHG and TPF images show patterns of collagen organization in the TM that match expected results with respect to both orientation and size. Through MPM, we intend to accurately determine the collagen fiber layer thickness, density, and orientation as a function of radial position and quadrant location.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years