Studies have shown that using high fluences of Low Intensity Laser Irradiation (HF-LILI) produce apoptotic effects on
normal and neoplastic cells. This study aimed to determine whether HF-LILI induce cell death in lung CSCs. Lung CSCs
were isolated using the stem cell marker CD 133, characterized using flow cytometry, and applied in experiments which
included treatment with LILI at wavelengths of 636, 825 and 1060 nm with fluences ranging from 5 J/cm2 to 40 J/cm2.
Viability and proliferation studies, using Alamar blue assay and adenosine triphosphate luminescence (ATP), indicated
an increase when treating lung CSCs with low fluences of 5 - 20 J/cm2 and a decrease in viability and proliferation as
well as an increase in apoptosis when applying a fluence of 40 J/cm2 indicated by flow cytometry using Annexin V and
propidium iodide (PI) dyes. Results indicate that LILI, when treating lung CSCs, can induce either a bio-stimulatory or
bio-inhibitory effect depending on the wavelength and fluence used. This study indicated successful apoptotic induction
of lung CSCs. Future experiments should be able to conclude the exact mechanism behind HF-LILI, which can be used
in the targeted treatments of CSC elimination, implementing HF-LILI in the same manner as PDT in the absence of a
photosensitizer.
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