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1 September 2007 Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source
Hiroyuki Yokoyama, Hiroshi Tsubokawa, Hengchang Guo, Junichi Shikata, Ki-ichi Sato, Keijiro Takashima, Kaori Kashiwagi, Naoaki Saito, Hirokazu Taniguchi, Hiromasa Ito
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Journal of Biomedical Optics, Vol. 12, Issue 5, 054019 (September 2007). https://doi.org/10.1117/1.2800393
Abstract
We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).
©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Hiroyuki Yokoyama, Hiroshi Tsubokawa, Hengchang Guo, Junichi Shikata, Ki-ichi Sato, Keijiro Takashima, Kaori Kashiwagi, Naoaki Saito, Hirokazu Taniguchi, and Hiromasa Ito "Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source," Journal of Biomedical Optics 12(5), 054019 (1 September 2007). https://doi.org/10.1117/1.2800393
Published: 1 September 2007
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Cited by 29 scholarly publications.
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KEYWORDS
Light
Green fluorescent protein
Luminescence
Semiconductor lasers
Fiber amplifiers
Picosecond phenomena
Light sources
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