Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-micron wavelength mode-locked semiconductor laser

Hengchang Guo; Keijiro Takashima; Aya Sato; Hiroyuki Yokoyama; Masahito Mure; Yuji Iseki; Hiroshi Tsubokawa

doi:10.1117/12.762922

15 February 2008 Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-μm wavelength mode-locked semiconductor laser

Hengchang Guo, Keijiro Takashima, Aya Sato, Hiroyuki Yokoyama, Masahito Mure, Yuji Iseki, Hiroshi Tsubokawa

Author Affiliations +

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 686020 (2008) https://doi.org/10.1117/12.762922
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We have developed a multi-kilowatt peak power 1-μm optical pulse source for two-photon microscopy. Utilizing an external-cavity hybrid mode-locked semiconductor laser, we were able to generate picosecond optical pulses at a 500-MHz repetition rate. With a semiconductor optical amplifier driven by synchronized electronic gating pulses, the optical pulse repetition rate was sub-harmonically extracted at 1-100 MHz. At a 10-MHz repetition rate, optical pulses were then amplified to a peak power of greater than 2 kW with a two-stage Yb-doped fiber amplifier. Using this light source, we successfully obtained clear two-photon images of mouse brain neurons expressing green fluorescent proteins.

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Hengchang Guo, Keijiro Takashima, Aya Sato, Hiroyuki Yokoyama, Masahito Mure, Yuji Iseki, and Hiroshi Tsubokawa "Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-μm wavelength mode-locked semiconductor laser", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686020 (15 February 2008); https://doi.org/10.1117/12.762922

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