Probing magnetic field effects on cellular redox state using autofluorescence intensity and lifetime microscopy

Kevin K. Tan; Carlos A. Renteria; Rishyashring R. Iyer; Alexander Ho; Janet E. Sorrells; Robert J. Usselman; Stephen A. Boppart

doi:10.1117/12.3003226

13 March 2024 Probing magnetic field effects on cellular redox state using autofluorescence intensity and lifetime microscopy

Kevin K. Tan, Carlos A. Renteria, Rishyashring R. Iyer, Alexander Ho, Janet E. Sorrells, Robert J. Usselman, Stephen A. Boppart

Author Affiliations +

Proceedings Volume PC12863, Quantum Effects and Measurement Techniques in Biology and Biophotonics; PC1286306 (2024) https://doi.org/10.1117/12.3003226
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Weak magnetic fields affect a multitude of biological processes including cell metabolism and are hypothesized to be a result of magnetic field-sensitive spin-selective radical-pair reactions. To provide much needed visualization of this process, we demonstrate the use of a custom-built multimodal nonlinear optical imaging system capable of measuring the redox state of cells through multi-photon-excited autofluorescence and autofluorescence lifetime of metabolic cofactors. We demonstrate a custom multi-axis Helmholtz coil system to apply time-varying magnetic fields across the sample during imaging. This imaging platform allows for characterization and optimization of the effects of magnetic fields on live cells and tissues.

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Kevin K. Tan, Carlos A. Renteria, Rishyashring R. Iyer, Alexander Ho, Janet E. Sorrells, Robert J. Usselman, and Stephen A. Boppart "Probing magnetic field effects on cellular redox state using autofluorescence intensity and lifetime microscopy", Proc. SPIE PC12863, Quantum Effects and Measurement Techniques in Biology and Biophotonics, PC1286306 (13 March 2024); https://doi.org/10.1117/12.3003226

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