Label-free polarimetric multimodal optical imaging of collagen

Lingxiao Yang; Rishyashring R. Iyer; Janet E. Sorrells; Eric J. Chaney; Stephen A. Boppart

doi:10.1117/12.3001241

13 March 2024 Label-free polarimetric multimodal optical imaging of collagen

Lingxiao Yang, Rishyashring R. Iyer, Janet E. Sorrells, Eric J. Chaney, Stephen A. Boppart

Proceedings Volume PC12834, Multimodal Biomedical Imaging XIX; PC1283402 (2024) https://doi.org/10.1117/12.3001241
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

To achieve a comprehensive optical characterization of collagen within its native environment, we present a novel label-free multimodal polarimetric imaging system that integrates polarization-sensitive second-harmonic generation microscopy, polarization-sensitive optical coherence microscopy, and two-photon autofluorescence lifetime imaging. Through simultaneous and co-registered measurements, this multimodal approach captures different collagen signatures by exploring diverse light-collagen interactions across multiple scales. Quantitative information was retrieved at the tissue level, the collagen fibril level, and the molecular level. We believe that this innovative multimodal and quantitative approach has the potential to significantly enhance our fundamental understanding of collagen, especially its intricate roles in human health and disease.

Conference Presentation

Citation Download Citation

Lingxiao Yang, Rishyashring R. Iyer, Janet E. Sorrells, Eric J. Chaney, and Stephen A. Boppart "Label-free polarimetric multimodal optical imaging of collagen", Proc. SPIE PC12834, Multimodal Biomedical Imaging XIX, PC1283402 (13 March 2024); https://doi.org/10.1117/12.3001241

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KEYWORDS

Collagen

Optical imaging

Polarimetry

Second harmonic generation

Tissues

Biological imaging

Imaging systems

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