Advances in wide-field camera-based time-correlated single photon counting fluorescence lifetime imaging

Klaus Suhling; Jakub Nedbal; Louis Obeid Mogridge; Shirwa Awale

doi:10.1117/12.3001927

13 March 2024 Advances in wide-field camera-based time-correlated single photon counting fluorescence lifetime imaging

Klaus Suhling, Jakub Nedbal, Louis Obeid Mogridge, Shirwa Awale

Proceedings Volume PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; PC1284808 (2024) https://doi.org/10.1117/12.3001927
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Camera-based time-correlated single photon counting (TCSPC) is a method where the position and the arrival time of the photons are recorded simultaneously. This has some advantages for fluorescence lifetime imaging (FLIM) with certain types of microscopy. It also allows FLIM with a macroscopic field of view. We report on nanosecond fluorescence lifetime imaging (FLIM) combined with total internal reflection fluorescence (TIRF) microscopy based on a 40 mm diameter crossed delay line anode detector. It has a few 100 picoseconds time resolution, and is read out via three standard TCSPC boards. We apply this wide-field TCSPC detector to identify Förster Resonance Energy Transfer (FRET) in cell membrane proteins in TIR-FLIM microscopy. In addition, we use a TCSPC single photon avalanche diode (SPAD) array as a macroscopic camera to visualise varnish removal on paintings.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Klaus Suhling, Jakub Nedbal, Louis Obeid Mogridge, and Shirwa Awale "Advances in wide-field camera-based time-correlated single photon counting fluorescence lifetime imaging", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC1284808 (13 March 2024); https://doi.org/10.1117/12.3001927

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Fluorescence lifetime imaging

Time correlated single photon counting

Cameras

Microscopy

Single photon avalanche diodes

Picosecond phenomena

Proteins

Show All Keywords

Keywords/Phrases

Search In:

Publication Years