Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

Klaus Suhling; Liisa M. Hirvonen; Wolfgang Becker; Stefan Smietana; Holger Netz; James Milnes; Thomas Conneely; Alix Le Marois; Ottmar Jagutzki

doi:10.1117/12.2227198

5 May 2016 Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

Klaus Suhling, Liisa M. Hirvonen, Wolfgang Becker, Stefan Smietana, Holger Netz, James Milnes, Thomas Conneely, Alix Le Marois, Ottmar Jagutzki

Author Affiliations +

Proceedings Volume 9858, Advanced Photon Counting Techniques X; 98580J (2016) https://doi.org/10.1117/12.2227198
Event: SPIE Commercial + Scientific Sensing and Imaging, 2016, Baltimore, MD, United States

Abstract

Time-correlated single photon counting (TCSPC) is a widely used, sensitive, precise, robust and mature technique to measure photon arrival times in applications such as fluorescence spectroscopy and microscopy, light detection and ranging (lidar) and optical tomography. Wide-field TCSPC detection techniques, where the position and the arrival time of the photons are recorded simultaneously, have seen several advances in the last few years, from the microsecond to the picosecond time scale. Here, we summarise some of our recent work in this field with emphasis on microsecond resolution phosphorescence lifetime imaging (PLIM) and nanosecond fluorescence lifetime imaging (FLIM) microscopy.

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Klaus Suhling, Liisa M. Hirvonen, Wolfgang Becker, Stefan Smietana, Holger Netz, James Milnes, Thomas Conneely, Alix Le Marois, and Ottmar Jagutzki "Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy", Proc. SPIE 9858, Advanced Photon Counting Techniques X, 98580J (5 May 2016); https://doi.org/10.1117/12.2227198

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