Maximizing the captured information of integral floating microscopy

Jong-Young Hong; Youngmo Jeong; Jonghyun Kim; Jiwoon Yeom; Byoungho Lee

doi:10.1117/12.2200947

15 October 2015 Maximizing the captured information of integral floating microscopy

Jong-Young Hong, Youngmo Jeong, Jonghyun Kim, Jiwoon Yeom, Byoungho Lee

Proceedings Volume 9672, AOPC 2015: Advanced Display Technology; and Micro/Nano Optical Imaging Technologies and Applications; 96720S (2015) https://doi.org/10.1117/12.2200947
Event: Applied Optics and Photonics China (AOPC2015), 2015, Beijing, China

Abstract

To capture the three-dimensional (3D) information of microscopic (micro) object, the light field microscopy (LFM) has been studied. A lens array is inserted into the conventional microscope and 3D information of micro object is captured in single shot. However, since the lateral resolution decreases severely because of lens array, the integral floating microscopy (IFM) is proposed. The IFM is modified version of the LFM which concentrates on the lateral resolution rather than the angular resolution by changing the location of specimen and the lens array. The specimen should be located at the front focal plane and the lens array should be located at the back focal plane of the objective lens in the IFM but it is hard to locate the lens array into the back focal length of the objective lens because the back focal length lies in the barrel of the objective lens in general. In this paper, we propose the modified version of the integral floating microscopy which can place the lens array at the optimum position. The structure of the whole system is changed and the relay lens is added to relay the back focal length outside. By placing the lens array at the optimum position, the captured information could be maximized, and by changing the focal length of the relay lens, the field of view (FOV) mismatch problem can be also mitigated. The relationship between the captured information and the specification of the system is analyzed and proper experiments are presented for the verification.

Citation Download Citation

Jong-Young Hong, Youngmo Jeong, Jonghyun Kim, Jiwoon Yeom, and Byoungho Lee "Maximizing the captured information of integral floating microscopy", Proc. SPIE 9672, AOPC 2015: Advanced Display Technology; and Micro/Nano Optical Imaging Technologies and Applications, 96720S (15 October 2015); https://doi.org/10.1117/12.2200947

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Relays

Objectives

Microscopy

Staring arrays

Spatial resolution

Charge-coupled devices

Confocal microscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years