Application of fluorescence spectroscopy and multispectral imaging for non-invasive estimation of GFP transfection efficiency

M. Tamošiūnas; D. Jakovels; A. Ļihačovs; A. Kilikevičius; J. Baltušnikas; R. Kadikis; S. Šatkauskas

doi:10.1117/12.2083895

22 October 2014 Application of fluorescence spectroscopy and multispectral imaging for non-invasive estimation of GFP transfection efficiency

M. Tamošiūnas, D. Jakovels, A. Ļihačovs, A. Kilikevičius, J. Baltušnikas, R. Kadikis, S. Šatkauskas

Author Affiliations +

Proceedings Volume 9421, Eighth International Conference on Advanced Optical Materials and Devices (AOMD-8); 94210M (2014) https://doi.org/10.1117/12.2083895
Event: Eighth International Conference on Advanced Optical Materials and Devices, 2014, Riga, Latvia

Abstract

Electroporation and ultrasound induced sonoporation has been showed to induce plasmid DNA transfection to the mice tibialis cranialis muscle. It offers new prospects for gene therapy and cancer treatment. However, numerous experimental data are still needed to deliver the plausible explanation of the mechanisms governing DNA electro- or sono-transfection, as well as to provide the updates on transfection protocols for transfection efficiency increase. In this study we aimed to apply non-invasive optical diagnostic methods for the real time evaluation of GFP transfection levels at the reduced costs for experimental apparatus and animal consumption. Our experimental set-up allowed monitoring of GFP levels in live mice tibialis cranialis muscle and provided the parameters for DNA transfection efficiency determination.

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M. Tamošiūnas, D. Jakovels, A. Ļihačovs, A. Kilikevičius, J. Baltušnikas, R. Kadikis, and S. Šatkauskas "Application of fluorescence spectroscopy and multispectral imaging for non-invasive estimation of GFP transfection efficiency", Proc. SPIE 9421, Eighth International Conference on Advanced Optical Materials and Devices (AOMD-8), 94210M (22 October 2014); https://doi.org/10.1117/12.2083895

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