pEGFP transfection into murine skeletal muscle by electrosonoporation

Mindaugas Tamošiūnas; Dainis Jakovels; Uldis Rubins; Roberts Kadikis; Ramona Petrovska; Saulius Šatkauskas

doi:10.1117/12.2297222

7 December 2017 pEGFP transfection into murine skeletal muscle by electrosonoporation

Mindaugas Tamošiūnas, Dainis Jakovels, Uldis Rubins, Roberts Kadikis, Ramona Petrovska, Saulius Šatkauskas

Proceedings Volume 10592, Biophotonics—Riga 2017; 105920L (2017) https://doi.org/10.1117/12.2297222
Event: The Second International Conference "Biophotonics-Riga 2017", 2017, Riga, Latvia

Abstract

In this study, we aimed to determine whether the combination of electroporation (EP) and ultrasound (US) waves (sonoporation) can affect the plasmid DNA transfection to mice tibialis cranialis muscle. Multispectral imaging technique combined with fluorescence spectroscopy point measurements has been used for the transcutaneous detection of enhanced green fluorescent protein (EGFP) fluorescence, providing information on location and duration of EGFP expression. We found that electrosonoporation, commonly enhancing pDNA transfection in vitro, had no positive effect on EGFP transfection efficiency increase in vivo with respect to electroporation alone. We presume that this may be associated with decreased viability of transfected fibers.

Citation Download Citation

Mindaugas Tamošiūnas, Dainis Jakovels, Uldis Rubins, Roberts Kadikis, Ramona Petrovska, and Saulius Šatkauskas "pEGFP transfection into murine skeletal muscle by electrosonoporation", Proc. SPIE 10592, Biophotonics—Riga 2017, 105920L (7 December 2017); https://doi.org/10.1117/12.2297222

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