Anti-translational research: from the bedside back to the bench for reflectance confocal microscopy

Daniel Gareau

doi:10.1117/12.2040504

4 March 2014 Anti-translational research: from the bedside back to the bench for reflectance confocal microscopy

Daniel Gareau

Proceedings Volume 8953, Optical Methods in Developmental Biology II; 89530M (2014) https://doi.org/10.1117/12.2040504
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The reflectance confocal microscope has made translational progress in dermatology. 0.5 micrometer lateral resolution, 0.75mm field-of-view and excellent temporal resolution at ~15 frames/second serve the VivaScope well in the clinic, but it may be overlooked in basic research. This work reviews high spatiotemporal confocal microscopy and presents images acquired of various samples: zebra fish embryo where melanocytes with excellent contrast overly the spinal column, chicken embryo, where myocardium is seen moving at 15 frames/ second, calcium spikes in dendrites (fluorescence mode) just beyond the temporal resolution, and human skin where blood cells race through the artereovenous microvasculature. For an introduction to confocal microscopy, see: http://dangareau.net.s69818.gridserver.com/science/confocal-microscopy

Citation Download Citation

Daniel Gareau "Anti-translational research: from the bedside back to the bench for reflectance confocal microscopy", Proc. SPIE 8953, Optical Methods in Developmental Biology II, 89530M (4 March 2014); https://doi.org/10.1117/12.2040504

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