Intra-vital microscopy of lung tissue: A simulation based analysis of the image formation

Maria Gaertner; Kerstin Schirrmann; Christian Schnabel; Sven Meissner; Ulrich Kertzscher; Lars Kirsten; Edmund Koch

doi:10.1117/12.2032814

18 June 2013 Intra-vital microscopy of lung tissue: A simulation based analysis of the image formation

Maria Gaertner, Kerstin Schirrmann, Christian Schnabel, Sven Meissner, Ulrich Kertzscher, Lars Kirsten, Edmund Koch

Proceedings Volume 8798, Clinical and Biomedical Spectroscopy and Imaging III; 87980G (2013) https://doi.org/10.1117/12.2032814
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

In the course of pulmonary research, understanding alveolar tissue dynamics plays a critical role in the treatment of patients suffering from acute lung diseases. As a gold standard technique for monitoring micro scale changes of lung tissue, real-time intra-vital microscopy (IVM) has been established to evaluate the behavior of the alveolar tissue. To allow profound qualitative and quantitative conclusions, characteristic features of the obtained images have to be thoroughly understood. These factors are strongly influenced by the imaging setup and physiological condition of the lung. To circumvent misinterpretations, a ray-tracing approach has been applied in this study using an idealized geometry of the mouse lung parenchyma deduced from optical coherence tomography (OCT) as a complementary imaging technique. Basic features of IVM images are double ring structures and disappearing of alveoli related to liquid infiltration. Ray propagation analysis reveals the formation of these features by two major reflection processes: partial reflection and total internal reflection. The results give rise to quantification errors of the alveolar area related to reflexes misinterpreted as alveolar borders and should further be used to yield a correction factor for future IVM lung tissue studies.

Citation Download Citation

Maria Gaertner, Kerstin Schirrmann, Christian Schnabel, Sven Meissner, Ulrich Kertzscher, Lars Kirsten, and Edmund Koch "Intra-vital microscopy of lung tissue: A simulation based analysis of the image formation", Proc. SPIE 8798, Clinical and Biomedical Spectroscopy and Imaging III, 87980G (18 June 2013); https://doi.org/10.1117/12.2032814

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available