Application of second-harmonic generation microscopy for in vivo observation of structural change in human dermal collagen fiber caused by aging and/or UV exposure

T. Yasui; M. Yonetsu; R. Tanaka; S. Fukushima; T. Yamashita; Y. Ogura; T. Hirao; T. Araki

doi:10.1117/12.907596

13 February 2012 Application of second-harmonic generation microscopy for in-vivo observation of structural change in human dermal collagen fiber caused by aging and/or UV exposure

T. Yasui, M. Yonetsu, R. Tanaka, S. Fukushima, T. Yamashita, Y. Ogura, T. Hirao, T. Araki

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Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 82263E (2012) https://doi.org/10.1117/12.907596
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Second-harmonic-generation (SHG) microscopy is useful to visualize collagen fiber in biological tissues in vivo. In this paper, we applied our SHG microscopy equipped with a Cr:Forsterite laser to visualize human dermal collagen fiber in vivo. The obtained SHG images indicated the structural difference of dermal collagen fiber between different ages, for example, fine collagen fiber is densely distributed in 20's subjects whereas only thick collagen fiber is remained in 60's subjects. These results reflect structural change of collagen fiber caused by natural aging and/or photoaging. The SHG microscopy has a potential to become an in vivo collagen-sensitive microscopy for assessment of skin aging.

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T. Yasui, M. Yonetsu, R. Tanaka, S. Fukushima, T. Yamashita, Y. Ogura, T. Hirao, and T. Araki "Application of second-harmonic generation microscopy for in-vivo observation of structural change in human dermal collagen fiber caused by aging and/or UV exposure", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82263E (13 February 2012); https://doi.org/10.1117/12.907596

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KEYWORDS

Collagen

Skin

Second-harmonic generation

Microscopy

In vivo imaging

Structured optical fibers

Visualization

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