Multiphoton spectroscopy of human skin in vivo

Hans G. Breunig; Martin Weinigel; Karsten König

doi:10.1117/12.908227

13 February 2012 Multiphoton spectroscopy of human skin in vivo

Hans G. Breunig, Martin Weinigel, Karsten König

Proceedings Volume 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X; 82251D (2012) https://doi.org/10.1117/12.908227
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

In vivo multiphoton-intensity images and emission spectra of human skin are reported. Optical sections from different depths of the epidermis and dermis have been measured with near-infrared laser-pulse excitation. While the intensity images reveal information on the morphology, the spectra show emission characteristics of main endogenous skin fluorophores like keratin, NAD(P)H, melanin, elastin and collagen as well as of second harmonic generation induced by the excitation-light interaction with the dermal collagen network.

Citation Download Citation

Hans G. Breunig, Martin Weinigel, and Karsten König "Multiphoton spectroscopy of human skin in vivo", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82251D (13 February 2012); https://doi.org/10.1117/12.908227

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