Excitation-wavelength dependence of multiphoton excitation of fluorophores of human skin in vivo

Hans Georg Breunig; Hauke Studier; Karsten König

doi:10.1117/12.840955

2 March 2010 Excitation-wavelength dependence of multiphoton excitation of fluorophores of human skin in vivo

Hans Georg Breunig, Hauke Studier, Karsten König

Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 754806 (2010) https://doi.org/10.1117/12.840955
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We present in vivo measurements of the excitation-wavelength dependence of the autofluorescence of major endogenous fluorophores of human skin with a multiphoton tomograph. For the investigation high-resolution multiphoton images at different depths inside the skin were recorded and the main fluorophores identified. In particular, for the autofluorescence of the fluorophores keratin, NAD(P)H, elastin and for the second-harmonic-generation light induced by collagen fibers clear trends are shown.

Citation Download Citation

Hans Georg Breunig, Hauke Studier, and Karsten König "Excitation-wavelength dependence of multiphoton excitation of fluorophores of human skin in vivo", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 754806 (2 March 2010); https://doi.org/10.1117/12.840955

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