Multicolor digital holographic microscope (DHM) for biological purposes

Zoltán Göröcs; Márton Kiss; Veronika Tóth; László Orzó; Szabolcs Tokés

doi:10.1117/12.841962

24 February 2010 Multicolor digital holographic microscope (DHM) for biological purposes

Zoltán Göröcs, Márton Kiss, Veronika Tóth, László Orzó, Szabolcs Tokés

Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 75681P (2010) https://doi.org/10.1117/12.841962
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Our digital holographic (DH) approach can be used to study tissue structures both in vitro and in vivo. This DHM architecture can produce three color microscopic 3D and 4D (video) images. We record 3 color (RGB) holograms with single exposures, and the perfect compensation of color crosstalk is solved. An in-line holographic setup and reconstruction algorithms are presented with demonstrative simulations and experimentally captured and numerically reconstructed images. Comparing the individually reconstructed color images with each other can provide information both for recognition of different types of cells or microorganisms, and for diagnostic purposes as well. Experimental example is given observing microscopic hydro-biological organisms using a color digital holographic microscope.

Citation Download Citation

Zoltán Göröcs, Márton Kiss, Veronika Tóth, László Orzó, and Szabolcs Tokés "Multicolor digital holographic microscope (DHM) for biological purposes", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75681P (24 February 2010); https://doi.org/10.1117/12.841962

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