A novel hyperspectral lifetime probe for autofluorescence

Pieter De Beule; C. Dunsby; D. M. Owen; N. P. Galletly; U. Anand; C. D. Benham; A. Naylor; G. W. Stamp; P. Anand; P. M. W. French

doi:10.1117/12.700590

14 February 2007 A novel hyperspectral lifetime probe for autofluorescence

Pieter De Beule, C. Dunsby, D. M. Owen, N. P. Galletly, U. Anand, C. D. Benham, A. Naylor, G. W. Stamp, P. Anand, P. M. W. French

Author Affiliations +

Proceedings Volume 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII; 643303 (2007) https://doi.org/10.1117/12.700590
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

The application of autofluorescence in non-invasive medical diagnostics could have great potential. Two major drawbacks inherent to this approach are low signal levels compared to those from exogenous fluorescent probes and complexity caused by the multiplicity of fluorescent biomolecules in tissue. Here we present a new optical system that is based on single channel detection via an optical fiber and can measure the fluorescence emission spectrum and fluorescence lifetime simultaneously for excitation wavelengths of 355 and 435nm. Single channel measurements integrate the signal normally available in an imaging setup and therefore have a better signal-tonoise ratio. Resolving both the fluorescence emission spectrum and fluorescence lifetime provides the opportunity to discriminate multiple fluorophores. This instrument is intended for NAD(P)H and flavin measurements for the dynamic monitoring of cellular metabolism and optical measurements of cancerous tissue. Initial results from a study of live cells and a clinical study of human skin lesions are presented.

Citation Download Citation

Pieter De Beule, C. Dunsby, D. M. Owen, N. P. Galletly, U. Anand, C. D. Benham, A. Naylor, G. W. Stamp, P. Anand, and P. M. W. French "A novel hyperspectral lifetime probe for autofluorescence", Proc. SPIE 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII, 643303 (14 February 2007); https://doi.org/10.1117/12.700590

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available