Two-photon fluorescence microscopy of in-vivo human skin

Rob F. M. Hendriks; Gerald W. Lucassen

doi:10.1117/12.410637

18 December 2000 Two-photon fluorescence microscopy of in-vivo human skin

Proceedings Volume 4164, Laser Microscopy; (2000) https://doi.org/10.1117/12.410637
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

We present images of human skin obtained by a two-photon fluorescence microscope, which has been optimized for in- vivo imaging. Using autofluorescence as a contrast mechanism various layers of the skin, e.g., stratum corneum, viable epidermis, basal layer and upper dermis could be clearly distinguished. A comparison between two-photon fluorescence images and reflectance images shows that there is a clear difference in the information content provided by both techniques. In particular, the fluorescence images of the dermis show detail that is absent in the reflectance images.

Citation Download Citation

Rob F. M. Hendriks and Gerald W. Lucassen "Two-photon fluorescence microscopy of in-vivo human skin", Proc. SPIE 4164, Laser Microscopy, (18 December 2000); https://doi.org/10.1117/12.410637

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