Three-dimensional confocal fluorescence microscopic visualization of the living human skin

Barry R. Masters

doi:10.1117/12.206820

12 April 1995 Three-dimensional confocal fluorescence microscopic visualization of the living human skin

Barry R. Masters

Proceedings Volume 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II; (1995) https://doi.org/10.1117/12.206820
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Three-dimensional confocal visualization of living human skin is a new development in the noninvasive imaging of normal and pathological tissue. I have investigated the autofluorescence of in vivo human skin with a laser scanning confocal microscope. An argon ion laser (488 nm) was used for excitation of the natural fluorescence of skin and a 515 nm cut off filter was used to separate the fluorescence from the excitation light. I found that normal skin has a very high autofluorescence. The laser scanning confocal microscope was used to obtain a stack of serial sections through the skin. A stack of optical sections through the hair follicle was reconstructed as well as the three-dimensional reconstruction of the pores of sweat glands. The ability to obtain two and three-dimensional visualizations of in vivo human skin may provide a new tool for noninvasive diagnostics in dermatology.

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Barry R. Masters "Three-dimensional confocal fluorescence microscopic visualization of the living human skin", Proc. SPIE 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II, (12 April 1995); https://doi.org/10.1117/12.206820

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KEYWORDS

Skin

Confocal microscopy

Microscopes

Laser scanners

In vivo imaging

Luminescence

Visualization

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