High-sensitivity determination of Zn(II) and Cu(II) in vitro by fluorescence polarization

Richard B. Thompson; Badri P. Maliwal; Vincent Feliccia; Carol A. Fierke

doi:10.1117/12.307337

10 April 1998 High-sensitivity determination of Zn(II) and Cu(II) in vitro by fluorescence polarization

Richard B. Thompson, Badri P. Maliwal, Vincent Feliccia, Carol A. Fierke

Proceedings Volume 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery; (1998) https://doi.org/10.1117/12.307337
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Recent work has suggested that free Cu(II) may play a role in syndromes such as Crohn's and Wilson's diseases, as well as being a pollutant toxic at low levels to shellfish and sheep. Similarly, Zn(II) has been implicated in some neural damage in the brain resulting from epilepsy and ischemia. Several high sensitivity methods exist for determining these ions in solution, including GFAAS, ICP-MS, ICP-ES, and electrochemical techniques. However, these techniques are generally slow and costly, require pretreatment of the sample, require complex instruments and skilled personnel, and are incapable of imaging at the cellular and subcellular level. To address these shortcomings we developed fluorescence polarization (anisotropy) biosensing methods for these ions which are very sensitivity, highly selective, require simple instrumentation and little pretreatment, and are inexpensive. Thus free Cu(II) or Zn(II) can be determined at picomolar levels by changes in fluorescence polarization, lifetime, or wavelength ratio using these methods; these techniques may be adapted to microscopy.

Citation Download Citation

Richard B. Thompson, Badri P. Maliwal, Vincent Feliccia, and Carol A. Fierke "High-sensitivity determination of Zn(II) and Cu(II) in vitro by fluorescence polarization", Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); https://doi.org/10.1117/12.307337

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