Internal fluorescent probes for studying irregular and metastable DNA structures

Theodore E. Carver; David P. Millar

doi:10.1117/12.182757

17 August 1994 Internal fluorescent probes for studying irregular and metastable DNA structures

Theodore E. Carver, David P. Millar

Proceedings Volume 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV; (1994) https://doi.org/10.1117/12.182757
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Time-resolved fluorescence anisotropy decay measurements are useful for characterizing differences in the local structural environment of probes attached to DNA molecules. We synthesized oligonucleotides with dansyl probes covalently attached to uridine near the center of a 17 base oligonucleotide. The fluorescence properties of these DNAs hybridized to complementary DNA were examined using time-correlated single photon counting. We present preliminary data indicating changes in the probe anisotropy in the presence of trifluoroethanol, divalent zinc cations, and under conditions promoting the formation of a hairpin junction at the site of probe incorporation. The results of these perturbations indicate that this approach will be useful for investigating site-specific and global structural phenomena in DNA.

Citation Download Citation

Theodore E. Carver and David P. Millar "Internal fluorescent probes for studying irregular and metastable DNA structures", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); https://doi.org/10.1117/12.182757

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