Indocyanine-green-assisted diode laser optoporation of plant cells

A. Pirolo; Marina Mazzoni; U. Vanni; Monica Monici; M. Buiatti; P. Bogani; Riccardo Pratesi

doi:10.1117/12.259968

26 November 1996 Indocyanine-green-assisted diode laser optoporation of plant cells

A. Pirolo, Marina Mazzoni, U. Vanni, Monica Monici, M. Buiatti, P. Bogani, Riccardo Pratesi

Proceedings Volume 2928, Biomedical Systems and Technologies; (1996) https://doi.org/10.1117/12.259968
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

A new technique for the absorption of cells is presented. A NIR diode laser (810 nm) and an absorption enhancing dye (indocyanine green, ICG) are used. Localized heating of ICG aggregates close to the cell surface generates bubbles inside the cells. Expansion and emergence of these bubbles cause the cells to open, thus creating the condition for successful transfection. Successful application to plant (tomato) cells is reported for the first time. The experimental results are directly linked both to the absorption characteristics of the dye employed and to the nature of the wall. Optoporation was achieved only by the use of the dye. Laser powers were employed comparable to those used for animal cells.

Citation Download Citation

A. Pirolo, Marina Mazzoni, U. Vanni, Monica Monici, M. Buiatti, P. Bogani, and Riccardo Pratesi "Indocyanine-green-assisted diode laser optoporation of plant cells", Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); https://doi.org/10.1117/12.259968

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